Cell-Type Specific Regulation of Dendritic mRNA in the Hippocampus

项目总结 这个职业发展奖的总体目标是获得必要的研究和专业技能。 需要建立一个外部资助的研究小组来研究转录后机制 潜在的社会记忆。具体地说，这笔赠款建立在以前在信使核糖核酸调控领域的培训基础上。 在神经元树突中，增加了基本和创新技术的培训，如生物信息学分析 和核糖体图谱，进一步在神经细胞类型中进行全基因组翻译调控-重要的 用于社交记忆。这笔赠款汇集了多名本地专家，以协同支持两个项目 职业和研究培训。将通过以下途径获得下一代测序分析的正式培训 小学的授课和实践教学。翻译侧写培训 方法和分析将在当地一所顶尖大学的联合导师的指导下教授。实验室 管理、资质和科学沟通技能将通过研讨会获得，简称 课程和在国家和区域科学会议上的演讲。最后，辅导技巧 将通过小学的暑期学生研究项目得到磨练。成功后 在完成赠款的培训部分后，预计获奖者将在 研究密集型大学的终身教职。 在奖项的独立阶段，研究计划扩大到包括基因靶向方法 询问特定细胞类型中的mRNA稳定性如何有助于突触和行为的可塑性 潜在的社会学习。这项拨款的工作将扩大我们对后转录的知识 调节神经元翻译的机制和潜在的确定新的候选治疗方法 具有社会认知异常加工的神经精神障碍的治疗，如自闭症谱系 精神障碍和精神分裂症。

PROJECT SUMMARY The overall goal of this career development award is to acquire the essential research and professional skills needed to establish an extramurally funded research group to investigate the post-transcription mechanisms underlying social memory. Specifically, the grant builds upon previous training in the field of mRNA regulation in neuronal dendrites and adds training in essential and innovative techniques, such as bioinformatics analyses and ribosome profiling, to further pursue genome-wide translational regulation in neuronal cell-types important for social memory. The grant brings together a number of local experts to synergistically support both the career and research training. Formal training in next generation sequencing analyses will be acquired through didactic coursework and hands-on instruction at the primary institution. Training in translational profiling methods and analyses will be taught under the guidance of a co-mentor at a local top university. Laboratory management, grantsmanship and scientific communication skills will be attained through workshops, short courses and presentations at national and regional scientific meetings, respectively. Lastly, mentorship skills will be honed through a summer student research program at the primary institution. Upon successful completion of the training portion of the grant, it is expected that the awardee will be highly competitive for a tenure-track faculty position at research-intensive university. During the independent phase of the award, the research plan expands to include a genetic targeting approach to interrogate how mRNA stability in specific cell-types contributes to synaptic and behavioral plasticity underlying social learning. Work from this grant will expand our knowledge of the post-transcriptional mechanisms regulating translation in neurons and potentially identify novel therapeutic candidates for the treatment of neuropsychiatric disorders with abnormal sociocognitive processing, such as autism spectrum disorders and schizophrenia.