Shared Resource-Advanced Biophotonics Core

先进生物光子学核心共享资源-项目摘要/摘要 高级生物光子学核心（ABC）提供成像和分析仪器， 和协作支持人员为先进的光学和电子显微镜的生物系统。癌 中心成员使用该设施进行高通量成像分析，活细胞的高分辨率成像， 组织动力学，超分辨率显微镜，组织的大3D体积成像，电子显微镜 亚细胞形态和蛋白质分布的分析，以及自动计算图像处理， 可视化和分析。ABC Core也在积极追求和开发新的尖端成像 和分析方法，以更好地满足癌症中心研究人员的需求，如冷冻相关 光学和电子显微镜，透明和扩张组织的光片成像，以及机器学习 基于光学和电子显微镜图像的处理、分割和分析。ABC核心是 致力于为癌症中心成员提供：1）获得光学和电子显微镜，专业 样品制备试剂和技术，以及用于分析的计算硬件和软件， 可视化，2）免费的一对一培训所有显微镜，以及图像处理和分析 软件，3）为成像实验设计和实施提供咨询和协作支持， 分析实验，4）用于电子显微镜检查的样品制备，组织清除和扩增 显微镜，5）先进的显微镜和软件技术的研讨会和演示，6）每周开放- 门成像靴子营先进的成像和图像处理技术，和7）每月 生物光子学科学研讨会系列，随后与ABC核心工作人员和用户进行市政厅式的讨论。

Advanced Biophotonics Core Shared Resource - Project Summary/Abstract The Advanced Biophotonics Core (ABC) provides imaging and analysis instrumentation coupled with technical and collaborative support staff for advanced light and electron microscopy of biological systems. Cancer Center members use the facility for high-throughput imaging assays, high-resolution imaging of live cell and tissue dynamics, super-resolution microscopy, large 3D volume imaging of tissues, electron microscopy analysis of subcellular morphology and protein distribution, and automated computational image processing, visualization, and analysis. The ABC Core is also actively pursuing and developing new cutting-edge imaging and analysis methodologies to better serve the needs of Cancer Center researchers, such as cryo-correlative light and electron microscopy, light-sheet imaging of cleared and expanded tissues, and machine-learning based processing, segmentation, and analysis of light and electron microscope images. The ABC Core is committed to providing Cancer Center members: 1) access to light and electron microscopes, specialized sample preparation reagents and technologies, and computational hardware and software for analysis and visualization, 2) free one-on-one training on all microscopes, as well as image processing and analysis software, 3) consulting and collaborative support for experimental design and implementation of imaging and analysis experiments, 4) sample preparation for electron microscopy, tissue clearing, and expansion microscopy, 5) workshops and demos with advanced microscope and software technologies, 6) weekly open- door imaging boot camp on advanced imaging and image processing techniques, and 7) a monthly Biophotonics scientific seminar series followed by town-hall style discussions with ABC Core staff and users.