Divergent Functional and Metabolic Development of the Infant Microbiome

梁摘要：项目2 减少早期生活环境中的微生物暴露和对婴儿肠道微生物群的干扰是 与儿童时期的哮喘结局有关。居住在墨西哥诺加莱斯的墨西哥人表现出四倍的 与居住70英里的同种血统人群相比，儿童哮喘发病率降低 在亚利桑那州图森市越过边境。我们的初步数据表明，这些跨境 人群在其环境(屋尘和饮酒)中经历了明显不同的微生物暴露 水)，在这些环境中长大的1个月大婴儿的粪便微生物区系差异丰富 对于免疫调节细菌属。这表明微生物在环境和个人方面的差异 微生物群与在这一人群中观察到的哮喘发病率的跨境差异有关。Beans提议 对居住在墨西哥诺加莱斯的墨西哥母婴二人组进行全面调查 亚利桑那州图森市及其早期生活环境的差异如何确定环境、母体和 婴儿微生物的组成和功能与跨境人群中哮喘的差异有关。项目2 将以高分辨率检查配对母亲产前子集的微生物组的功能属性 (阴道和粪便)和婴儿肠道(纵向收集的粪便)样本来自200个母婴二人组， 以及他们配对的房屋灰尘和水样本，使用猎枪元基因组学，RNA-Seq和 代谢组学。这项研究将检查遗传微生物和肠道微生物组的功能差异 跨境社区中与2岁时T2喘息相关的发展(哮喘的有力预测因子 在儿童后期)和免疫发育。这些努力旨在确定环境、产妇和婴儿 微生物组的属性在墨西哥人口中促进对哮喘的保护。

BEAMS ABSTRACT: Project 2 Diminished early life environmental microbial exposures and perturbations to the infant gut microbiome are related to asthma outcomes in childhood. Mexican populations resident in Nogales, Mexico exhibit a four-fold decrease in childhood asthma rates compared with a population of comparable ancestry resident seventy miles away across the border in Tucson, Arizona. Our preliminary data has demonstrated that these cross-border populations experience significantly distinct microbial exposures in their environment (house dust and drinking water) and that the stool microbiota of 1-month old infants raised in these environments are differentially enriched for immunomodulatory bacterial genera. This suggests that microbial differentials in environmental and personal microbiomes relate to the cross-border disparity in asthma rates observed in this population. BEAMS proposes to comprehensively examine a population of Mexican mother-infant dyads resident in Nogales, Mexico or Tucson, Arizona and their environment in early life to determine how differences in environmental, maternal and infant microbial composition and function relate to asthma disparities in this cross-border population. Project 2 will examine at high-resolution the functional attributes of the microbiome of a subset of paired maternal prenatal (vaginal and stool) and infant gut (longitudinally collected stool) samples collected from 200 mother-infant dyads, as well as their paired house dust and water samples using shotgun metagenomics, RNA-Seq and metabolomics. The study will examine functional differentials in inherited microbes and gut microbiome development in cross-border communities that relate to T2 wheezing at age 2 years (a robust predictor of asthma in later childhood) and immune development. These efforts aim to identify environmental, maternal and infant microbiome attributes that promote protection against asthma within Mexican populations.