Flow Cytometry Shared Resource

流式细胞仪共享资源 项目摘要 流式细胞仪共享资源(FCSR)的目标是为维克森林浸礼会提供综合 癌症中心(WFBCCC)成员可随时获得必要的专业知识和最先进的流程 可广泛应用于癌症生物学研究的细胞学仪器。流式细胞术是一种 提供快速单细胞分析和细胞分类的关键技术 光散射特性和单个细胞内或细胞上多个细胞分子或代谢物的存在。 此外，FCSR寻求促进协议的开发，这些协议可以利用不断增加的 可用标记和方法的数量。FCSR的重点是解决两者的需求 经验丰富的流式细胞仪用户以及那些刚刚发现流式细胞仪技术功能的用户。 为此，每月为新用户提供仪器培训，并在实验中提供支持 培训后的设计和数据解释。可用的仪器包括BD FACS Aria细胞分选仪 (三个激光和十一个参数)，BD FACS Canto II分析仪(三个激光和十个参数)和BD FACS、卡利伯和Accuri分析仪(两台激光和六个参数)。除了每个流程中的软件 细胞仪，额外的分析软件可用于分析多参数数据以识别和表征 细胞亚群。DNA直方图分析软件程序也可供需要的研究人员使用 先进的细胞周期分析。FCSR由Martha Alexander-Miller博士领导，并设有 1.33 FTE的工作人员。为了实现该设施的目标，FCSR有以下具体目标：1) 为流式细胞仪分析提供尖端的分析仪器；2)提供多参数细胞 对用户定义的人群进行分类；3)为新用户和现有用户提供仪器培训；以及4)提供 在实验设计和流式细胞仪数据解释方面的专业知识。2014年11月-10月 2015赠款年度，FCSR为42个用户提供服务，其中74%是WFBCCC成员； 那些，%有同行评议的资金。WFBCCC会员占营业时间的85%。

FLOW CYTOMETRY SHARED RESOURCE Project Summary The goal of the Flow Cytometry Shared Resource (FCSR) is to provide Wake Forest Baptist Comprehensive Cancer Center (WFBCCC) members with ready access to the necessary expertise and state-of-the-art flow cytometry instrumentation that can be applied to a wide range of cancer biology research. Flow cytometry is a critical technology that provides rapid single-cell analysis and cell sorting through the simultaneous detection of light scatter properties and the presence of multiple cellular molecules or metabolites in or on an individual cell. In addition, the FCSR seeks to foster the development of protocols that can utilize the continually increasing number of available markers and methodologies. The FCSR is focused on addressing the needs of both seasoned flow cytometry users as well as those just discovering the capabilities of flow cytometry technology. To this end, monthly training for the instruments is provided for new users as well as support in experimental design and data interpretation following training. Instruments available include a BD FACS Aria cell sorter (three lasers and eleven parameters), a BD FACS Canto II analyzer (three lasers and ten parameters) and BD FACS Calibur and Accuri analyzers (two lasers and six parameters). In addition to the software with each flow cytometer, additional analysis software is available to analyze multiparameter data to identify and characterize cell subpopulations. DNA histogram analysis software programs are also available for researchers requiring advanced cell cycle analysis. The FCSR is under the direction of Martha Alexander-Miller, Ph.D., and has a staff of 1.33 FTEs. To accomplish the goals of the facility, the FCSR has the following Specific Aims to: 1) Provide cutting-edge analysis instrumentation for flow cytometric analysis; 2) Provide multi-parameter cell sorting for user-defined populations; 3) Provide instrument training for new and existing users; and 4) Provide expertise for experimental design and interpretation of flow cytometry data. In the November 2014 – October 2015 grant year, the FCSR provided services to forty-two users, 74% of whom were WFBCCC members; of those, 64% had peer-reviewed funding. WFBCCC members accounted for 85% of the service hours.