Phase II SER-CAT Optimization: Acquisition of a next generation area detector

说明(由申请者提供)：申请资金用于为东南地区协作访问小组(SER-CAT)22区高级光子源(www.ser-cat.org)购买下一代高速X射线探测器，作为22ID的第二阶段优化。SER-CAT成立于1997年，现已发展到包括来自14个州25个机构的90多个研究小组。SER-CAT光束线提供数据和结构，以支持(1)联邦资助和其他研究活动，(2)工业药物发现和(3)新提案所需的初步结果。Ser-CAT的成功体现在其首屈一指的光束线22ID已成为世界上生产效率最高的大分子结晶学设备之一。SER-CAT成员机构认为，SER-CAT对于他们的结构生物学项目以及在吸引(和留住)最优秀和最聪明的教职员工和学生方面至关重要。每个机构必须支付年度会费(根据其资本支出份额)来维护设施和接收束流时间，这一事实反映了SER-CAT对其计划的重要性。提议购买下一代FAST探测器的目的是：(1)目前在22ID上使用的探测器已有五年以上的历史，接近可靠性问题。因此，良好的管理要求SER-CAT积极主动地确保其成员能够在其主要光束线上获得可靠的大幅面CCD探测器。(2)目前的提议是我们计划优化SER-CAT数据收集能力的第二阶段。拟议的探测器将最大限度地提高第一阶段微衍射仪采集的影响，方法是提高来自每周衍射微晶体的数据的信噪比，我们的成员经常发现很难从SER-CAT收集数据。(3)拟议的Rayonix MX325-HS-100T探测器(其读数为0.097秒)将使22ID每天可进行的实验次数增加一倍或三倍，这反过来将增加外部资金的成功(即更多的项目获得更多的束流时间，产生更多的结果)。(4)所提出的探测器将允许我们将22ID上的当前MX300探测器移动到SER-CAT弯曲磁束线路22BM。这一举措将显著增加可在22BM上进行的实验范围。重要的是，在22ID探测器发生故障时，MX300探测器将作为22ID的必要备份，而MAR 225安装在22BM上。Ser-CAT目前支持美国国立卫生研究院资助的80多个研究项目，这些项目旨在产生与理解蛋白质结构-功能关系及其作用机制相关的蛋白质三维结构。这些研究将显著影响我们对细胞过程的了解，并为疾病的分子基础提供有价值的见解，从而带来更好的治疗方法。

DESCRIPTION (provided by applicant): Funds are requested for the purchase of a next generation, high-speed X-ray detector for the Southeast Regional Collaborative Access Team (SER-CAT) Sector 22, Advanced Photon Source, Argonne National Laboratory (www.ser-cat.org), as the Phase II optimization of 22ID. Organized in 1997, SER-CAT has grown to include over 90 research groups from 25 institutions spanning 14 states. The SER-CAT beam lines provide data and structures in support of (1) federally funded and other research activities, (2) industrial drug discovery and (3) preliminary results needed for new proposals. SER-CAT's success is reflected by the fact that its premiere beam line 22ID has become one of the world's most productive facilities for macromolecular crystallography. SER-CAT member institutions view SER-CAT to be critical to their structural biology programs and in attracting (and retaining) the best and brightest faculty and students. The fact that each institution must pay yearly dues (based on their share of capital expense) to maintain the facility and to receive beam time reflects the importance of SER-CAT to their programs. The aims of the proposed acquisition of a next generation, fast detector are: (1) the current detector in use on 22ID is now over five years old and nearing the point where reliability can become an issue. Thus, good management requires that SER-CAT be proactive in assuring its membership access to a reliable large format CCD detector on its premier beam line. (2) The current proposal represents Phase II of our planned optimization of data collection capability at SER-CAT. The proposed detector will maximize the impact of the Phase I microdiffractometer acquisition by improving the signal-to-nose ratio for data from weekly diffracting micro crystals that our members often find difficult to collect data from at SER-CAT. (3) The proposed Rayonix MX325-HS-100T detector (with its 0.097 sec readout) should double or triple the number of experiments that can be carried out per day on 22ID, which in-turn should increase the success of extramural funding (i.e. more projects get beam time producing more results). (4) The proposed detector will allow us to move the current MX300 detector on 22ID to the SER-CAT bending magnet beam line 22BM. This move will significantly increase the range of experiments that can be carried out on 22BM. importantly; the MX300 detector will serve as a needed backup for 22ID with the MAR 225 installed back on 22BM, in the event of 22ID detector failure. SER-CAT currently supports over 80 NIH funded research projects aimed at producing three-dimensional structures of proteins related to understanding protein structure-function relationships and their mechanism of action. These studies will significantly impact our knowledge of cellular processes and provide valuable insights into the molecular basis of disease that will lead to better therapeutics.