Acquisition of a 800 MHz NMR Spectrometer

描述（由申请人提供）：我们建议通过获得一台能够进行溶液和固态核磁共振实验的800 MHz核磁共振光谱仪来扩大华盛顿大学（UW）的核磁共振资源。近年来，西雅图地区从事生物固态和/或生物溶液核磁共振独立或合作的研究小组数量急剧增加。获得超高场核磁共振设备（即>600 MHz）的需求至少按比例增加。就在几年前，生物固态核磁共振（ssNMR）是由一个主要用户（Drobny）和一两个次要用户（Stayton和后来的Castner）合作完成的。现在，该提案的三个主要用户（Drobny, Klevit, Varani）都有正在进行的固态核磁共振项目。UW的单个DMX-750设备不适合做固态核磁共振，而且无论如何，UW的DMX-750上没有足够的ssmr时间来容纳三个主要用户和几个次要用户的项目。同样，华盛顿大学和弗雷德·哈钦森癌症研究中心正在进行的生物大分子溶液核磁共振研究的研究小组数量也有所增加，以至于西雅图地区的任何领域都没有足够的核磁共振能力来满足这一需求，由于DMX-750磁铁的技术限制，这种情况尤其严重，这阻碍了它进行长期的高分辨率核磁共振实验。因此，即使是主要用户Klevit和Varani也必须在PNNL和其他地方申请大量的光谱仪时间。西雅图地区的许多团队不得不前往偏远地区使用超高场核磁共振设备，或者在机会出现时只能满足于等待当地使用低场核磁共振仪器，这表明我们需要扩大西雅图地区超高场核磁共振的能力。我们恭敬地请求NIH为我们提供资金，这样我们就可以获得一个固态/溶液800 MHz光谱仪，以满足我们主要和次要用户的当前需求。

DESCRIPTION (provided by applicant): We propose to expand the NMR resources at the University of Washington (UW) by acquiring a 800 MHz NMR spectrometer capable of performing both solution and solid state NMR experiments. The number of research groups in the Seattle area involved in performing biological solid state and/or biological solution NMR independently or collaboratively has increased dramatically in recent years. The need for access to ultra-high field NMR equipment (i.e. >600 MHz) has increased at least proportionally. Only a few years ago biological solid state NMR (ssNMR) was performed by a single major user (Drobny) and collaboratively by one or two minor users (Stayton and later Castner). Now all three major users on this proposal (Drobny, Klevit, Varani) have ongoing projects in solid state NMR. The single DMX-750 at UW is poorly equipped to do solid state NMR and in any case not enough ssNMR time is available on the DMX-750 at UW to accommodate the projects of three major users and several minor users. Likewise the number of research groups at UW and at the Fred Hutchinson Cancer Research Center with on-going solution NMR studies of biological macromolecules has also increased to the point that there is simply not enough NMR capacity at any field in the Seattle area to meet this demand, a situation especially serious because of the technical limitations of the DMX-750 magnet, which prevent it from performing long-run high resolution NMR experiments. Therefore, even major users Klevit and Varani must apply for large slots of spectrometer time at PNNL and elsewhere. That so many groups in the Seattle area have to travel to remote locations to access ultra-high field NMR equipment or have to be content to await access locally to lower field NMR instruments as the opportunity arises, demonstrates that we need to expand the capacity to do ultra-high field NMR in the Seattle area. We respectfully request that NIH provide the funding to us so we can acquire a solid state/solution 800 MHz spectrometer that will serve the current needs of our major and minor users. PUBLIC HEALTH RELEVANCE: Knowledge of the structure and dynamics of biomolecules is fundamental to understanding their function and is therefore a vital component of our ability to understand and treat human disease. Solid state and solution NMR spectroscopies are able to elucidate structure and dynamics of biological macromolecules in solution, in microcrystalline and amorphous solid form, adsorbed onto biomaterial surfaces or oriented in lipid bilayers. Members of our user group are funded predominantly by NIH and to some extent also by NSF to use NMR and other methods to study a wide variety of proteins and nucleic acids, with relevance to developing treatments for a variety of cancers, heart disease, HIV, and optimizing the performance and design of biomedical implants, biosensors, and engineered tissues.