Echo 555 Acoustical Droplet Ejection System for High-throughput Screening

描述（由申请人提供）：UT西南高通量筛选（HTS）实验室创建并进行小分子筛选，以确定那些可以成为开发治疗方法或用作研究工具的线索。此外，HTS实验室进行基因组RNAi筛选，以确定开发治疗方法的新靶点。HTS实验室管理着UTSW化学化合物文件，目前包括超过20万种类似药物的小有机化合物。这个复合图书馆是由机构和NIH基金购买的，免费提供给已经批准项目的UT西南研究人员。HTS实验室帮助UT西南研究人员开发能够以HTS格式进行筛选的检测方法，无论是由HTS实验室在当地进行筛选，还是作为MLPCN的一部分。HTS实验室筛选、分析、收集和分析筛选数据。HTS实验室进行二级筛选，以验证在初级筛选实验中确定的感兴趣的化合物。HTS实验室的科学家与nci资助项目的研究人员、Simmons综合癌症中心的成员或其他nih资助的研究人员合作，对HTS实验中发现的感兴趣的化合物进行优先排序，并管理这些化合物的补充和储存。HTS核心维护一个筛选数据的Oracle数据库，以便任何HTS实验中感兴趣的化合物的活性可以与使用UTSW化合物文件进行的所有其他HTS实验进行比较。除了进行HTS筛选外，HTS实验室还培训并建议西南大学的学生、博士后、工作人员和教职员工进行HTS实验的开发和执行。

DESCRIPTION (provided by applicant): The UT Southwestern high-throughput screening (HTS) laboratory creates and conducts screens of small molecules to identify those that can become leads for developing therapeutics or used as tools in research. In addition, the HTS laboratory conducts genomic RNAi screens to identify novel targets for the development of therapeutics. The HTS laboratory manages the UTSW chemical compound file currently including more than 200,000 drug-like small organic compounds. This compound library, which was purchased with institutional and NIH funds, is provided at no cost to UT Southwestern Investigators who have approved projects. The HTS laboratory aids UT Southwestern investigators in developing assays that are capable of being screened in HTS format, either locally by the HTS laboratory or as part of the MLPCN. The HTS laboratory screens assays and collects and analyzes screening data. The HTS lab conducts secondary screens required to validate compounds of interest identified during primary screening experiments. Working with the investigators of a NCI-funded Program Project, members of the Simmons Comprehensive Cancer Center, or other NIH-funded investigators, scientists in the HTS laboratory prioritize the compounds of interest identified in HTS experiments and manage resupply and storage of those compounds. The HTS core maintains an Oracle database of screening data so that the activity of compounds of interest in any HTS experiment can be compared to all other HTS experiments conducted with the UTSW compound file. In addition to conducting HTS screens, the HTS laboratory trains and advises students, postdoctoral fellows, staff scientists and faculty at UT Southwestern on the development and execution of HTS experiments.