Multi-color Flow Analyzer for the UGA-CTEGD Flow Facility

描述（由申请人提供）：本提案要求为热带和新兴全球疾病中心/佐治亚大学流式细胞仪核心设施购买一台新的CyAn ADP 9彩色流式细胞仪。虽然位于UGA热带和新兴全球疾病中心内，并由该中心运营和维护，该中心是美国主要的热带疾病研究中心之一，但UGA的CTEGD流动设施为整个大学社区以及众多当地生物企业和区域学术机构提供服务。目前设备中分析仪的使用超出了容量，特别是用于高端多色（bbb4色）分析，这正在成为流式细胞术的标准，特别是在免疫学相关研究中。我们现在的青安已经7岁多了，即使在清晨、晚上和周末，她也被安排得很满。购买新的青安将在正常工作时间为用户提供更多的时间（当新用户需要培训和监督时），并在非工作时间产生容量，防止必须在非固定样品上完成的工作计划延迟，当我们当前的青安（现在已经7年了）停机时提供备用>4色分析功能，并提供适应我们不断扩大的用户群的能力。

DESCRIPTION (provided by applicant): This proposal requests funds to purchase a new CyAn ADP 9 color Flow Cytometry Analyzer for the Center for Tropical and Emerging Global Disease/University of Georgia Flow Cytometry Core Facility. Although housed within and operated and maintained by the UGA Center for Tropical and Emerging Global Diseases, one of the premier centers for tropical diseases research in the United States, the CTEGD Flow Facility at UGA serves the entire university community as well as numerous local bio-businesses and regional academic institutions. Usage of the current analyzers in the facility exceeds capacity - especially for higher end multi-color (>4 color) analysis - which is becoming a norm in flow cytometry, particularly in immunology-related research. Our current CyAn, now more than 7 years old is heavily scheduled even in the very early mornings, evenings and weekends. Purchase of a new CyAn will provide more user time during normal business hours (when training and oversight is necessary for newer users) as well generate capacity during off hours, prevent scheduling delays for work that must be done on non-fixed samples, provide back-up >4-color analysis capabilities when our current CyAn (now 7 years old) is down, and the provide the ability to accommodate our continuously expanding user base. PUBLIC HEALTH RELEVANCE: The majority of the research to be conducted using the proposed flow analyzer will focus on avariety of pathogens that collectively infect 100's of millions of individuals and are responsible for the annual deaths of millions. We seek to understand the basic biology, biochemistry, and pathogenesis of these organisms as well as the immune response to them. In addition to serving the CTEGD, the Flow Cytometry Facility serves a much broader academic and industry community with a diverse range of interests and needs in other areas of infectious diseases, stem cell research, cancer biology and developmental biology.