FEI Tecnai G2 Spirit BioTWIN Transmission Electron Microscope

描述（由申请人提供）：一个由27名美国国立卫生研究院资助的研究人员组成的小组在加州大学圣地亚哥分校申请资金购买FEI Tecnai G2 Spirit BioTWIN透射电子显微镜（TEM），以继续满足细胞和分子医学系（CMM）、医学院和加州大学圣地亚哥分校生物系内各种研究人员目前和未来的需求。所要求的TEM具有针对生物样品的高对比度显微镜优化的物镜配置，并且非常适合成像传统的超薄塑料切片，免疫金标记的超薄冷冻切片和阴性染色样品。Tecnai系统特别适用于不同的用户群体和多用户环境。该部目前使用了20年的仪器显示出明显的磨损迹象，并且由于仪器的使用年限和过去的使用情况，停机时间越来越长。未来的可靠性是一个主要问题，我们正在寻求用现代化的、最先进的仪器取代其中一个tem。美国国立卫生研究院资助的研究人员研究了各种各样的项目，从单细胞生物到膜运输，以及血管重塑，器官发生和宿主细菌相互作用中的信号传导。他们的工作跨越了从细胞和分子生物学到免疫学、病理学、神经科学、发育生物学和传染病的各种学科。它们都有一个共同的事实，即它们以细胞生物学问题为中心，需要超微结构分析和免疫定位研究。新的TEM将确保我们优秀的研究人员团队能够持续和不间断地获得这些领域最先进的研究所需的技术和应用。FEI Tecnai G2 Spirit BioTWIN将位于现有的CMM透射电子显微镜设施中，进行管理和维护，这是众多研究人员定期使用的共享资源。新的临时费用管理办法将列入现有的费用回收制度，以确保部分回收用于薪金、维修协议和用品的资金。

DESCRIPTION (provided by applicant): A group of 27 NIH funded investigators at UCSD requests funds to purchase a FEI Tecnai G2 Spirit BioTWIN transmission electron microscope (TEM) to continue to meet the ongoing and future demands of a variety of researchers within the Department of Cellular and Molecular Medicine (CMM), within the School of Medicine, and within the Division of Biology at UCSD. The requested TEM has an objective lens configuration optimized for high contrast microscopy of biological samples and is perfectly suited for imaging conventional ultrathin plastic sections, immunogold labeled ultrathin cryosections, and negatively stained samples. The Tecnai system is particularly adaptable to a diverse group of users and a multi-user environment. The Department's current 20 year old instruments are showing clear signs of wear and are plagued by increasing down time due to the age and past usage of the instruments. Future reliability is a major concern, and we are seeking to replace one of the TEMs with a modern, state-of-the-art instrument. Our NIH funded investigators study a variety of projects, ranging from single cell organisms to membrane trafficking, and signaling during vascular remodeling, organogenesis and host bacterial interactions. Their work spans a wide variety of disciplines from cell and molecular biology to immunology, pathology, neuroscience, developmental biology and infectious disease. All have in common the fact that they center on cell biological questions that require ultrastructural analysis and immunolocalization studies. The new TEM will ensure that our group of outstanding investigators have continued and uninterrupted access to the techniques and applications necessary for state-of-the-art research in these fields. The FEI Tecnai G2 Spirit BioTWIN will be located, administered and maintained in the existing CMM Transmission Electron Microscopy Facility -- a shared resource that is utilized regularly by numerous researchers. The new TEM will be included in the existing cost recovery system to ensure partial recovery of funds for salaries, maintenance agreements and supplies.