DEVELOPMENT OF FERRET REAGENTS FOR USE IN THE CHARACTERIZATION OF IMMUNE RESPONSES TO RESPIRATORY INFECTIONS IN THE FERRET MODEL.

雪貂是甲型流感病毒、SARS冠状病毒和MERS冠状病毒等人类呼吸道病毒的优秀模型。这项提议的目标是产生抗体，用于分析雪貂在呼吸道感染期间的免疫细胞和细胞因子反应。主要的焦点是免疫细胞标志物和这些细胞在免疫反应过程中产生的细胞因子。承包商计划使用标准杂交瘤技术来产生单抗，然后使用各种方法进行广泛的免疫学鉴定，以显示重组免疫原以及选定的雪貂样本的特异性。

Ferrets represent excellent models of human respiratory viruses such as influenza A virus, SARS-CoV and MERS-CoV. The goal of this proposal is to generate antibodies for analyzing immune cells and cytokine responses during respiratory infections in ferrets. The main focuses are immune cell markers and cytokines produced by these cells during immune responses. The contractor plans to use standard hybridoma techniques to generated monoclonal antibodies will then undergo extensive immunological characterization using various methods to show the specificity to recombinant immunogens as well as selected ferret samples.