Histopathology and Stone Analysis

项目摘要 核心B将对项目1、2和3进行所有的形态和病理分析。乳头评分是 PPG的重要组成部分，核心B将分析项目1和项目2的所有手术记录，以量化 所有乳头上的菌斑和堵塞区域，并确保整个项目的乳头评分是一致的。 项目2将从仔细分类的结石患者身上收集人体组织，核心B将 在活检材料上使用专门的染色进行光学和电子显微镜(光镜和电子显微镜)检查，以及 使用显微红外光谱(FT-IR)和拉曼光谱方法鉴定组织中的矿物质。核心B将 还要准备项目3用于激光显微解剖的冰冻切片，以及 免疫组织化学染色切片以验证活检内容。在项目1和2中收集的所有石头都将被分析 通过Micro CT和常规FT-IR提供关于石头类型和矿物百分比的详细信息 内容。B芯还将对锂进行原子吸收光谱分析，以确定远端 在研究患者中输送溶质(项目1)，以及在组织标本中输送钙(项目3)。

Project Summary Core B will conduct all the morphological and pathology analyses for Projects 1, 2, and 3. Papillary scoring is an essential part of the PPG, and Core B will analyze all surgical recordings from Projects 1 and 2 to quantify plaque and plug areas on all papillae and to ensure that papillary scoring across the projects is consistent. Human tissues will be collected by Project 2 from carefully categorized stone patients, and Core B will perform light and electron microscopy (LM and EM) using specialized stains on the biopsy material, and using micro- infrared spectroscopy (FT-IR) and Raman methods to identify mineral in the tissue. Core B will also prepare the frozen sections to be used by Project 3 for laser micro-dissection, along with immunostained sections to verify the biopsy content. All stones collected in Projects 1 and 2 will be analyzed by micro CT and conventional FT-IR to provide detailed information on stone type and percent mineral content. Core B will also perform atomic absorption spectrophotometric analysis of lithium to determine distal delivery of solutes in study patients (Project 1), and of calcium in tissue specimens (Project 3).