Northwestern University Skin Biology and Diseases Resource-based Center

西北大学(NU)因其强大的跨学科皮肤生物学研究项目而受到认可 在与表皮结构和功能相关的研究中具有特别强的实力。利用这一优势， NU皮肤生物与疾病资源中心(SBDRC)的主题是“角质形成细胞及其 微环境“，其目标是促进优秀的翻译研究。NU SBDRC拥有41个 高级法官、11名初级法官和12名临床协作组成员， 包括13个大学系和医学系内的5个司。信息 通过SBDRC产生的数据最终将改善患者的护理。为了实现这一目标，SBDRC已经 以下核心：(I)行政(行政)；(Ii)皮肤组织工程和形态(STEM)；(Iii) 翻译和实验性皮肤测试和免疫示踪(测试IT)；和(Iv)基因编辑， 转导和纳米技术(进入)。管理核心鼓励SBDRC之间的合作 高级和初级BASE研究成员以及与合作的临床助理成员 通过它的浓缩计划。在浓缩计划范围内，SBDRC将支持其试点和 可行性(P&F)计划，将在新的方向上扩展皮肤研究，并成为新的来源 来自怒族社区的SBDRC成员。管理核心将以其少数族裔和性别意识(MSA)为特色 该计划促进了研究，以阐明基于性别和肤色的皮肤差异。STEM CORE将提供：(I)正常角质形成细胞、成纤维细胞和黑素细胞，患者皮损皮肤细胞，以及 诱导多能干细胞(IPS)，包括广泛的细胞库；(Ii)人体器官型三维培养 和小鼠表皮；(Iii)角质形成细胞共培养，包括与免疫细胞和神经；和(Iv)一个 广泛的形态发生加工服务。测试IT核心侧重于重要的 角质形成细胞和皮肤免疫系统之间的关系，并提供仪器和 免疫检测服务，以及在受控环境中进行人体测试的独特机会。 Get in Core将产生：(I)慢病毒和逆转录病毒构建物，以过度表达或沉默基因并 在皮肤细胞中表达报告；(Ii)产生和分化IPSCs的病毒；(Iii)CRISPR/Cas结构 用于导入角质形成细胞；(Iv)用于传递Cas9和引导RNA的非病毒方法；以及(V)a 使用纳米技术的各种其他非病毒传递技术(球形核酸/SNAs，高密度 脂蛋白/类高密度脂蛋白纳米颗粒)和基于纳米喷雾探针的电穿孔。跟踪、测试IT并获得 在核心相互交错和其他大学核心，通过培训和提供教育 对结果的解释，并具有很强的翻译成分。NU SBDRC的理想位置是 为用户提供将他们的基本发现应用到临床环境中的机会，这将使Nu Skin 生物学家在了解和治疗皮肤病方面取得了重要进展。

Northwestern University (NU) is recognized for its strong interdisciplinary cutaneous biology research program with particular strength in investigations related to epidermal structure and function. Leveraging this strength, the theme of the NU Skin Biology and Disease Resource Center (SBDRC) is the “Keratinocyte and its Microenviroment”, with its goal to promote outstanding translational research. The NU SBDRC, with its 41 Senior Bench members, 11 Junior Bench members and 12 Collaborating Clinical Associate members, encompasses 13 University departments and 5 divisions within the Department of Medicine. Information generated through the SBDRC will ultimately improve patient care. To accomplish this goal, the SBDRC has the following Cores: (i) Administrative (Admin); (ii) Skin Tissue Engineering and Morphology (STEM); (iii) Translational and Experimental Skin Testing and Immune Tracing (TEST IT); and (iv) Gene Editing, Transduction and Nanotechnology (GET iN). The Admin Core encourages collaboration among the SBDRC Senior and Junior Bench Research members, as well as with the Collaborating Clinical Associate members through its Enrichment Program. Within the Enrichment Program, the SBDRC will support its Pilot and Feasibility (P&F) Program, which will extend cutaneous research in new directions and be a source of new SBDRC members from the NU community. The Admin Core will feature its Minority and Sex Awareness (MSA) Program, which promotes research to elucidate the differences in skin based on sex and skin color. The STEM Core will provide: (i) normal keratinocytes, fibroblasts, and melanocytes, cells from patient lesional skin, and induced pluripotent stem (iPS) cells, including an extensive cell bank; (ii) 3D organotypic cultures of human and mouse epidermis; (iii) keratinocyte co-cultures, including with immune cells and nerves; and (iv) an extensive array of morphogenetic processing services. The TEST IT Core focuses on the important relationship between keratinocytes and the skin immune system, and provides instrumentation and immunological assay services, as well as the unique opportunity for human testing in a controlled environment. The GET iN Core will generate: (i) lentiviral and retroviral constructs to overexpress or silence genes and to express reporters in skin cells; (ii) viruses for generating and differentiating iPSCs; (iii) CRISPR/Cas constructs for introduction into keratinocytes; (iv) nonviral approaches for delivering CAS9 and guide RNAs; and (v) a variety of other nonviral delivery techniques using nanotechnology (spherical nucleic acids/SNAs, high density lipoprotein/HDL-like nanoparticles) and Nanofountain Probe-based Electroporation. STEM, TEST IT and GET iN cores interdigitate with each other and other university cores, provide education through training and interpretation of results, and have strong translational components. The NU SBDRC is ideally positioned to provide users with opportunities to take their basic findings into a clinical setting, which will enable NU skin biologists to make important strides in understanding and treating diseases of the skin.