Translational Nanobiology Approaches to SARS-CoV2

具体目标1：测试NCI转化纳米生物学技术用于SARS-CoV 2和COVID-19的研究。通过在NINDS病毒免疫学部分利用BSL-2+区域进行NIH N-VITA研究，并通过测试NCI转化纳米生物学部分开发的专用工具和方法，以促进病毒和COVID-19相关细胞外囊泡中蛋白质和RNA货物的超灵敏检测，我们寻求进一步表征来自COVID-19患者的SARS-CoV 2病毒和细胞外囊泡（EV）库。具体目标2：通过我们的部门网站nano.ccr.cancer.gov建立一个可公开访问的EV和病毒流式细胞术方案和工具集合。我们的目的是在nano.ccr.cancer.gov上建立一个开放访问资源，其中NIH N-VITA计划中开发的所有病毒和EV检测或表征方案和方法将与全球研究社区共享。自网站推出以来，我们几乎每周都会发布更新。

Specific Aim 1: Test NCI Translational Nanobiology technologies for use in the study of SARS-CoV2 and COVID-19. By utilizing a BSL-2+ area for NIH N-VITA studies in the NINDS Viral Immunology Section, and by testing specialized tools and methods developed in the NCI Translational Nanobiology Section to facilitate ultra-sensitive detection of protein and RNA cargo in the viruses and in COVID-19 associated extracellular vesicles, we seek to advance characterization of both SARS-CoV2 viruses and Extracellular Vesicle (EV) repertoires from patients with COVID-19. Specific Aim 2: Establish a publicly accessible collection of protocols and tools for EV and Viral flow cytometry via our section website, nano.ccr.cancer.gov. Our intention with this effort is to establish an open-access resource at nano.ccr.cancer.gov wherein all protocols and methods for virus and EV detection or characterization that is developed in this NIH N-VITA program will be shared with the worldwide research community. Since launch of the website, we have been posting updates on a near-weekly basis.