Single clone discovery using an image-based cell isolation platform

摘要 一组细胞之间的功能和遗传异质性的特征 免疫学、癌症研究、神经生物学和 发育生物学。分离具有相同表型的细胞是了解 如此的异质性。我们想要开发一种非流体的、独特的技术，LCD 显微镜下的辅助选择(LASUM，图1)以满足这一需求。在.期间 在这个项目的第一阶段，我们完成了原型设计的阿尔法版本和 在三个概念验证实验中证明了它的有效性：(A)去除血细胞；(B) 噬菌体在单珠上的浓缩；(C)抗肿瘤免疫细胞克隆的分离。我们 愿意继续努力将这项低成本的技术推向市场， 高吞吐量、防碎片、基于图像，操作简单，因为准备 用显微镜载玻片或清洗微量滴定板。除了设备，我们还将统一 使用两个相关试剂盒的碎片化细胞分离市场：丰富-实时和丰富- 序列号。该设备/套件的成功商业化将使成千上万的细胞- 生物实验室和医院在计算机屏幕上操纵单个细胞。

Abstract Characterizing both functional and genetic heterogeneity among a pool of cells remains a major scientific challenge in immunology, cancer research, neurobiology and developmental biology. Isolating cells with the same phenotype is key to understand such heterogeneity. We would like to develop a non-fluidic, unique technology, LCD aided selection under microscope (LASUM, Fig.1) to address this need. During the phase I of this project, we completed the alpha version of prototype design and demonstrated its utility in three proof-of-concept experiments: (a) blood cell removal; (b) phage enrichment on single beads; (c) isolation of tumor-killing immune cell clones. We would like to continue our effort to bring this technology to market, which will be low-cost, high throughput, debris resistant, image-based with operation simplicity as preparing a microscope slide or washing a microtiter plate. Besides the device, we will unify the fragmentated cell isolation market using two associated kits: Enrich-Live and Enrich- Seq. The successful commercialization of this device/kits will enable thousands of cell- biology labs and hospitals in manipulating single cells on a computer screen.