Live Cell Imaging Shared Resource

Harold C.西蒙斯癌症中心 活细胞成像共享资源（转化研究资源） 项目总结/摘要 活细胞成像共享资源维护并提供10台光学显微镜和3台 图像处理工作站显微镜的范围从相对简单的仪器，用于拍摄照片， 组织学染色的样本，以先进的双光子显微镜成像活动物和厚 组织中受过训练的，值得信赖的用户可以使用显微镜，每小时收费。设施工作人员提供个人 培训和援助。该资源是服务于整个UT西南的机构核心设施 研究社区。癌症中心会员占设施使用者的50%以上， 每小时使用的仪器。资源主任和设施经理提供个人培训 免费向癌症中心的设施用户提供帮助，而向会员收取服务费 根据癌症中心提供的支持量减少。 服务还包括协议开发建议，实验故障排除和帮助 成像数据的适当解释和有效呈现。希望使用该设施的调查人员 通常与导演会面，讨论哪种显微镜最适合他们的项目， 确定样品制备和实验设计的适当方案。主任担任 在需要常规显微镜成像以外的项目上担任顾问或合作者。设施经理 培训新用户。为了最大限度地提高培训的效率和效果，用户在实际标本上接受培训 他们将被成像，并且只被教导与他们的实验有关的特征。设施经理是 在正常工作时间内，为用户提供帮助。经批准的用户可以在下班后签出钥匙 access.一个基于网络的预约系统被用来预约时间。它与用户帐户集成 数据库和用于跟踪实际使用情况的认证服务器。

Harold C. Simmons Cancer Center Live Cell Imaging Shared Resource (Translational Research Resource) Project Summary/Abstract The Live Cell Imaging Shared Resource maintains and provides access to ten light microscopes and three image processing workstations. The microscopes range from relatively simple instruments for taking pictures of histologically stained samples to sophisticated two photon microscopes for imaging in live animals and thick tissues. Trained, trusted users may use the microscopes for an hourly fee. Facility staff provides individual training and assistance. The resource is an institutional core facility serving the whole UT Southwestern research community. Cancer Center members account for more than 50% of the users of the facility and 70% of the hourly use of the instruments. The Resource Director and Facility Manager provide individual training and assistance to Cancer Center users of the facility at no cost, while fees charged to members for services are reduced according to the amount of support provided by Cancer Center. Services also include advice on protocol development, troubleshooting of experiments and help with the appropriate interpretation and effective presentation of imaging data. Investigators who wish to use the facility typically meet with the Director to discuss which microscope would be most suitable for their project and to determine appropriate protocols for sample preparation and experimental design. The Director serves as consultant or collaborator on projects requiring more than routine microscope imaging. The facility manager trains new users. To maximize the efficiency and efficacy of training, users are trained on the actual specimens they will be imaging and are only taught the features that pertain to their experiment. The facility manager is available during normal working hours to assist users. Approved users may check out a key for after-hours access. A web-based calendaring system is used to reserve time. It is integrated with a user accounts database and an authentication server for tracking actual usage.