Single Cell Analysis Core (Core C)

项目摘要/摘要-核心C：单细胞分析核心 HR是由细胞间的遗传和代谢过程的变异引起的。单细胞分析的目标 核心(核心C)是提供经过实验验证但难以执行的单细胞级别分析 评估人力资源的机制和动态。核心C将支持所有建议的单细胞水平的研究 三个项目，为控制良好的抗生素下的细菌细胞培养提供集中平台 环境，以单细胞分辨率测量相关过程，并执行图像分析以及 必要的统计分析。具体来说，核心C将i)提供微流控连续培养平台，ii) 量化抗性基因的拷贝数变异，以及iii)测定单细胞在 异型抗性种群。核心C活动将由金敏秀博士在该部门的实验室进行 在单细胞水平的成像和基因分析方面拥有丰富的专业知识 细菌的表达、新陈代谢和生理学。金博士是首批应用单细胞技术的科学家之一 他致力于抗生素研究，被认为是该领域的领先专家。金博士和他的团队将 与单个项目的PI协作，确保他们能够及时成功地完成项目 举止。

PROJECT SUMMARY/ABSTRACT - CORE C: Single-Cell Analysis Core HR arises from cell-to-cell variation of genotypic and metabolic processes. The goal of the Single-Cell Analysis Core (Core C) is to provide experimentally-validated and yet difficult-to-perform single-cell-level analyses to evaluate the mechanisms and dynamics of HR. Core C will support the single-cell-level studies proposed in all three Projects by providing a centralized platform for culturing bacterial cells under well-controlled antibiotic environments, measuring relevant processes at single-cell resolution, and performing image analysis as well as necessary statistical analysis. Specifically, Core C will i) provide microfluidic continuous culture platform, ii) quantify the copy number variation of resistance genes, and iii) determine metabolic activities of single cells in heteroresistant populations. Core C activities will be conducted by Dr. Minsu Kim's laboratory in the Department of Physics at Emory University, which has extensive expertise in single-cell-level imaging and analysis of gene expression, metabolism, and physiology in bacteria. Dr. Kim was one of the first scientists to apply a single-cell approach to antibiotic research and is considered a leading expert in this area. Dr. Kim and his team will work with the PI of individual projects collaboratively to ensure they can successfully complete their projects in a timely manner.