Cytometry Core

流式细胞术是一种重要的工具来量化和表型细胞，定义相关的途径糖尿病。 以前仅限于循环免疫细胞的分析，从组织中提取细胞的进展， 允许流式细胞术将其能力扩展到发育，代谢和信号传导的研究 在各种各样的细胞类型的程序。细胞计数和细胞分选核心（CCSC）是 哥伦比亚大学糖尿病研究中心（DRC），因为它解决了许多DRC的研究需求， 研究糖尿病的免疫、发育和代谢方面的研究者。芯丝在 2010年与哥伦比亚转化免疫学中心和免疫学系合作创建， 医学，大量的大学投资以及NIH的支持（S10和P30赠款），并在 2017年通过整合癌症中心的流式细胞术核心。核心从5台扩展到11台 （4台来自癌症中心，2台新的光谱流式细胞仪）提供了更多的选择， 刚果民主共和国的灵活性。我们预计刚果民主共和国成员对核心的使用将保持稳定和强劲， 有吸引力的新技术提供了光谱流式细胞术。CCSC将以两种方式协助研究者： 目的1 -定量和表型表征有助于代谢的细胞群， 糖尿病及其并发症的免疫学和发育程序。CCSC将利用先进的 单细胞分辨率的荧光成像技术，以支持不同细胞类型的分析， 人和动物组织，包括1型糖尿病的人源化小鼠模型。目的2 -净化 与糖尿病及其并发症相关的细胞群。使用荧光激活细胞分选， CCSC将支持刚果民主共和国的研究人员纯化用于体内培养的单个细胞群 植入或分子表征，包括遗传和转录谱分析、蛋白质纯化 和信号传导研究，以及用于功能分析，包括T细胞活化、增殖和迁移研究。 为达致上述目标，公务员事务委员会已制定标准作业程序，以：（i）确保品质控制 和再现性;（ii）优先考虑研究者使用;（iii）监测核心使用情况;（iv）适应新技术 和哥伦比亚研究基地的需要。

Flow cytometry is an important tool to quantify and phenotype cells that define pathways relevant to diabetes. Previously limited to the analysis of circulating immune cells, advances in cell extraction from tissues has allowed flow cytometry to expand its capabilities to the study of developmental, metabolic and signaling programs in a wide variety of cell types. The Cytometry & Cell Sorting Core (CCSC) is a vital component of the Columbia University Diabetes Research Center (DRC), as it addresses the research needs of many DRC investigators who study immunological, developmental, and metabolic aspects of diabetes. The Core was created in 2010 in partnership with the Columbia Center for Translational Immunology and the Department of Medicine, with substantial University investment as well as NIH support (S10 and P30 grants) and expanded in 2017 by incorporating the Cancer Center’s flow cytometry core. The core expansion from 5 to 11 instruments (4 coming from the Cancer Center and 2 new spectral flow cytometers) has provided greater choice and flexibility for DRC members. We expect DRC member usage of the core to remain steady and strong, given attractive new technologies afforded by spectral flow cytometry. CCSC will assist investigators in two ways: Aim 1 – To quantify and phenotypically characterize cell populations that contribute to the metabolic, immunological, and developmental programs of diabetes and its complications. CCSC will leverage advanced technologies in fluorescent imaging at the single cell resolution to support the analysis of different cell types in human and animal tissues, including humanized mouse models of Type 1 diabetes. Aim 2 – To purify populations of cells of relevance to diabetes and its complications. Using fluorescence-activated cell sorting, the CCSC will support DRC investigators to purify individual populations of cells for culture, in vivo implantation, or molecular characterization including genetic and transcriptional profiling, protein purification and signaling studies, and for functional analysis including T cell activation, proliferation, and migration studies. To achieve these aims, the CCSC has established standard operating procedures to: (i) assure quality control and reproducibility; (ii) prioritize investigator use; (iii) monitor Core usage; and (iv) adapt to new technologies and to the needs of the Columbia Research Base.