Ocular Phenotyping Core

UAB视觉研究中心核心--眼部表型核心 项目/摘要摘要 眼结构和视功能的评估对于视觉基础研究和翻译研究都是必不可少的 无论是在动物模型上还是在人类受试者身上。广泛使用的技术包括光学相干 用于显示后段和前段分层组织的断层扫描(OCT)、视网膜电流图(ERG， 用于可分成多个可识别成分的块状视网膜信号)，用于前部生物显微镜的裂隙灯 和后段，通过多种可视化模式(彩色、自发荧光、染料- 基于血管造影术、红外反射)和视动性眼球震颤(评估视觉运动控制、视力 和对比敏感度)。为了应对日益增长的UAB视力研究人员的需求，“视觉表型核心” 已建立，以包含一套全面的工具并提供必要的支持 进行准确的眼部表型鉴定。具体仪器包括Bioptigen 840 nm SD-OCT和Micron IV Digital 小动物的眼底相机，小动物包括大鼠和小鼠的视力性视动性眼震 斑马鱼，和光谱SDOCT为大型动物和人类供体眼睛。授予保罗博士的S10奖助金 Gamlin在2020年允许购买三种新工具，这三种工具将极大地增强两者的眼睛表型 大小动物组织1)蔡司Lumera 700用Resight 700手术显微镜重新扫描 术中光学相干断层扫描(OCT)成像；2)FLEX模块SpectralisOCT2系统 用OCT对不同动物的视网膜和视神经头进行结构和血管造影成像 3)前段CASIA SS-1000扫描源OCT(Tomey Corp.)对角膜、虹膜进行成像， 大型动物的晶状体，以及小动物的整个眼睛。 该核心将支持15名UAB Vision科学家，其中包括13名计划中等到广泛的科学家 目前由NEI R01资助。这一拟议核心的主任和协理主任发表了大量出版物 分别具有视网膜电描记术和OCT验证/解释经验。核心区的新方向 将包括完成一个新的基于LED的ERG系统，该系统将简化这一测试。此外，一个 现有的AOSLO系统最初是为人类视网膜成像而设计的，将进行重新设计，以成像 活体中的啮齿动物和树鼠的眼睛。同时，荧光成像和细胞靶向的能力 将增加光刺激。此外，还将发布眼睛表型鉴定机会的广告，以确定新的 通过全眼表型屏幕建立眼部小鼠模型的非眼部小鼠模型 科学家，他们的动物最初是用来回答与他们的器官系统有关的问题的 利息。

UAB Center Core for Vision Research - Ocular Phenotyping Core Project/Summary Abstract Assessment of ocular structures and visual function is essential to both basic and translational research in vision science whether in animal models or human subjects. Widely used technologies include optical coherence tomography (OCT) for display of layered tissues in posterior and anterior segments, electroretinography (ERG, for massed retinal signals separable into multiple identified components), slit lamp for bio-microscopy of anterior and posterior segment, imaging of the fundus via multiple modes of visualization (color, autofluorescence, dye- based angiography, infrared reflectance), and optokinetic nystagmus (to assess visuomotor control, visual acuity and contrast sensitivity). In response to growing UAB vision researcher needs, the “Ocular Phenotyping Core” was established to encompass a comprehensive suite of instrumentation and to provide the necessary support for accurate ocular phenotyping. Specific instruments include Bioptigen 840 nm SD-OCT and Micron IV digital fundus camera for small animals, Optomotry optokinetic nystagmus in small animals including rats, mice and zebrafish, and Spectralis SDOCT for large animals and human donor eyes. An S10 grant awarded to Dr. Paul Gamlin in 2020 has allowed the purchase of three new tools that will greatly enhance ocular phenotyping of both large and small animal tissues 1) Zeiss Lumera 700 ReScan with Resight 700 operating microscope with intraoperative optical coherence tomography (OCT) imaging; 2) FLEX Module Spectralis OCT2 System for imaging the retina and optic nerve head with structural and angiographic OCT, in animals at various body positions; 3) Anterior Segment CASIA SS-1000 swept source OCT (Tomey Corp.) for imaging the cornea, iris, and lens in large animals, as well as the whole eye in small animals. This core will support 15 UAB Vision Scientists, including 13 with planned moderate to extensive all of whom are currently NEI R01-funded. The Director and Associate Director of this proposed core have extensive publication experience in electroretinography and OCT validation/ interpretation, respectively. New directions for the core will include the completion of a new LED-based ERG system that will simplify this testing. Further, an existing AOSLO system originally designed for human retinal imaging will be re-engineered in order to image rodent and tree shrew eyes in vivo. At the same time, capabilities for fluorescence imaging and cell-targeted photo-stimulation will be added. Additionally, Ocular Phenotyping Opportunities will be advertised to identify new ocular mouse models through full ocular phenotyping screens of mouse models generated by non-ocular scientists whose animals were originally generated to answer questions pertinent to their organ systems of interest.