Mays Cancer Center at UT Health SA

流式细胞仪共享资源项目总结 流式细胞术共享资源（FCSR）自2009年以来一直是癌症中心支持的共享资源。 1991. FCSR的使命是使用细胞分选仪进行高通量、多维分析和细胞分选。 为Mays癌症中心（MCC）成员提供最先进的设备，德克萨斯大学健康圣安东尼奥 (UT卫生局）调查人员和圣安东尼奥地区的其他人员。FCSR占地800多平方米。英尺的 实验室空间，方便MCC成员访问。FCSR服务包括多参数 细胞亚群的分析和分选;细胞周期分析;下游单细胞克隆和分选 基因组学/转录组学;活性氮和氧（NOS和ROS）、pH、Ca++的测量 不同活体染料的通量和通量;线粒体损伤的研究;以及 半胱天冬酶FCSR还提供咨询服务，包括实验设计，仪器培训， 协助赠款和手稿写作（包括准备数字，方法部分和预算）。 FCSR为MCC成员提供了两台流式细胞仪细胞分选仪、四台常规分析仪、一台 光谱分析仪和最近的质谱细胞仪。FCSR由科学总监Michael Berton领导， 哲学博士、微生物学、免疫学和分子遗传学副教授。Karla Gorena女士是一位 公司技术总监兼日常运营经理。姓名Kirma，博士，副教授 分子医学，于2018年加入FCSR担任第二位技术总监，负责监督Helios质量 细胞计数器。FCSR由UT Health SA副总统办公室提供行政支持， 研究（VPR）和MCC。在2018年，35个MCC成员与同行评审的资金使用FCSR。在 此外，在上一个报告期（2014-2018年），FCSR为53份癌症相关出版物提供了资料。

Project Summary—Flow Cytometry Shared Resource The Flow Cytometry Shared Resource (FCSR) has been a Cancer Center-supported Shared Resource since 1991. The mission of the FCSR is to deliver high-throughput, multi-dimensional analysis and cell sorting using state-of-the-art equipment for Mays Cancer Center (MCC) members, University of Texas Health San Antonio (UT Health SA) investigators, and others in the San Antonio area. The FCSR occupies over 800 sq. ft. of laboratory space and is conveniently accessible by MCC members. FCSR services include multiparameter analysis and sorting of subpopulations of cells; cell cycle analysis; single cell cloning and sorting for downstream genomics/transcriptomics; measurements of reactive nitrogen and oxygen species (NOS and ROS), pH, Ca++ fluxes and fluxes of different vital dyes; study of mitochondrial damage; and determination of activated caspases. The FCSR also offers consultative services including experimental design, instrument training, and assistance with grant and manuscript writing (including preparation of figures, methods sections, and budgets). The FCSR provides MCC members access to two flow cytometry cell sorters, four conventional analyzers, a spectral analyzer and, most recently, a mass cytometer. The FCSR is led by Scientific Director Michael Berton, Ph.D., Associate Professor of Microbiology, Immunology & Molecular Genetics. Ms. Karla Gorena is a Technical Director and daily operations manager of the FCSR. Nameer Kirma, Ph.D., Associate Professor of Molecular Medicine, joined the FCSR as a second Technical Director in 2018 to supervise the Helios mass cytometer. The FCSR is administratively supported by the UT Health SA Office of the Vice President for Research (VPR) and the MCC. During 2018, 35 MCC members with peer-reviewed funding used the FCSR. In addition, the FCSR contributed to 53 cancer-related publications during the last reporting period (2014-2018).