Development of an Automated Diagnostic Platform for SARS-CoV-2 Monitoring in Vulnerable Areas

项目摘要 新出现的具有快速传播能力的病原体（如SARS-CoV-2）造成的破坏， 证明了为未来病毒爆发做好准备的重要性;这包括快速 部署现场检测工具和流行病学监测， 特别是在最容易成为传染源的地方。 我们建议开发一个多功能的检测平台，SARS-CoV-2唾液。拟议 系统将整合来自不同生物识别元件的信号。适体、抗体和ACE 2将被 固定在激光雕刻的石墨烯电极上，以及针对SARS-CoV-2的检测机制 将在不同检测条件（pH值、温度、离子强度）下研究加标蛋白。后 确定每个生物传感器的增强性能的操作条件， 将在互补识别元件之间使用方法（即，可能组合 适体、抗体和ACE 2酶生物传感器）以评估对测试结果准确性的影响（即， 假阳性和假阴性结果的风险）。将制定唾液预处理方案， 促进使用多重生物传感器平台在人唾液中检测SARS-CoV-2。的开放通道 微流体系统将被设计成自动分裂和通道一个唾液样本到多个 流到生物传感器而没有饱和、生物污染和泵要求。的发展 功能和可靠的多重生物传感器系统将有助于解决公众的长期需求 随着呼吸道冠状病毒家族继续其季节性访问，这可能会重复几次 几十年来，很像流感病毒偶尔来访，具有不同程度的毒性。

Project Summary The devastation caused by emerging pathogens with fast transmission capacity, such as SARS-CoV-2, has demonstrated the importance of preparedness for future viral outbreaks; this includes the ability for fast deployment of in-situ testing tools and epidemiological surveillance with high temporal and spatial resolution; particularly in places that are most vulnerable to becoming reservoirs of infectious agents. We propose to develop a versatile multiplexing detection platform for SARS-CoV-2 in saliva. The proposed system will integrate signals from different biorecognition elements. Aptamer, antibody, and ACE2 will be immobilized onto laser inscribed graphene electrodes, and detection mechanisms targeting SARS-CoV-2 spike protein will be studied under varying testing conditions (pH, temperature, ionic strength). After determining the operating conditions for enhanced performance of each biosensor, a self-referencing approach will be used between complementary recognition elements (i.e., possible combinations of aptamers, antibodies, and ACE2 enzyme biosensors) to evaluate the effects on test results accuracy (i.e., risk of false-positive and false-negative results). A saliva pre-treatment protocol will be developed to facilitate SARS-CoV-2 testing in human saliva using the multiplex biosensor platform. An open channel microfluidics system will be designed to automatically split and channel a single saliva sample into multiple streams to the biosensors without saturation, biofouling, and pump requirement. The development of a functional and reliable multiplex biosensor system will be useful for addressing longstanding needs in public health as the respiratory Coronavirus family continues its seasonal visits, which may repeat over several decades, much like the occasional visits from the influenza virus, with varying degrees of virulence.