Monitoring antimicrobial resistance among veterinary pathogens at Iowa State University Veterinary Diagnostic Laboratory: A Vet-LIRN AMR Surveillance Project

项目摘要/摘要 爱荷华州立大学兽医病原体耐药性监测 兽医诊断实验室：VET-LIRN AMR监测项目 抗菌素耐药性(AMR)是影响人类福祉的主要健康威胁， 动物和世界范围内的环境。在美国，兽医实验室 食品和药物管理局下属的调查和反应网络(VET-LIRN)， 兽医中心(FDA-CVM)在选定的兽医中进行AMR监测 细菌病原体(如沙门氏菌、大肠埃希氏菌和假中间葡萄球菌) 通过与北美各地的诊断实验室合作，从动物身上分离出来。在.之下 倡议，爱荷华州立大学兽医诊断实验室(ISU VDL)成为一名兽医- LIRN源实验室，并参与了AMR监视计划 从2017年开始。FDA Vet-LIRN计划通过以下方式支持网络实验室 关于研究和监测用品、设备、人员和 扩大实验室能力和能力所需的其他相关活动。作为以下内容的一部分 在这个项目中，ISU VDL收集细菌分离物并将它们发送到基因组测序 在另一个地方的实验室。在此应用程序中，我们建议继续协作 与Vet-LIRN合作，以实现AMR监视计划的动态目标 在FOA PAR-18-604“VET-LIRN网络能力建设项目”中有部分描述。 具体来说，作为源头实验室，我们将继续分离和鉴定细菌病原体 在ISU VDL感兴趣的，执行抗菌素敏感性测试，并分享分离株和 Vet-LIRN网络每季度一次的药敏结果如下 VET-LIRN协议。我们坚信，执行支助股兽医诊断实验室已经 大大促进了#年AMR监测项目的能力和有效性 在过去的几年里，我们对兽医病原体产生了很大的兴趣，并愿意继续我们现有的伙伴关系 与FDA Vet-LIRN合作，以帮助全国/全球打击 兽医环境中的抗菌素耐药性。

Project Summary/Abstract Monitoring antimicrobial resistance among veterinary pathogens at Iowa State University Veterinary Diagnostic Laboratory: A Vet-LIRN AMR Surveillance Project Antimicrobial resistance (AMR) is a major health threat affecting the well-being of humans, animals, and the environment worldwide. In the United States, Veterinary Laboratory Investigation and Response Network (Vet-LIRN) under the Food and Drug Administration, Center for Veterinary Medicine (FDA-CVM) performs surveillance of AMR in select veterinary bacterial pathogens (e.g., Salmonella, Escherichia coli, and Staphylococcus pseudintermedius) from animals by partnering with diagnostic laboratories throughout North America. Under the initiative, Iowa State University Veterinary Diagnostic Laboratory (ISU VDL) became a Vet- LIRN source laboratory and has participated the AMR surveillance program from the very beginning since 2017. The FDA Vet-LIRN program supports the network laboratories via cooperative agreements for research and surveillance for supplies, equipment, personnel and other related activities that are needed to expand laboratories’ capability and capacity. As part of this project, the ISU VDL collects bacterial isolates and sends them to a Genome Sequencing laboratory in another location. In this application, we propose to continue the collaborative partnership with Vet-LIRN to address the dynamic goals of the AMR Surveillance Program as partly described in FOA PAR-18-604 “Vet-LIRN Network Capacity-Building Projects”. Specifically, as a source laboratory, we will continue to isolate and identify bacterial pathogens of interests at ISU VDL, perform antimicrobial susceptibility testing, and share the isolates and antimicrobial susceptibility testing result with Vet-LIRN network on a quarterly basis following the Vet-LIRN protocol. We strongly believe that the ISU Veterinary Diagnostic Laboratory has substantially contributed to the capacity and effectiveness of the AMR surveillance project in veterinary pathogens over the past years, and would like to continue our existing partnership with the FDA Vet-LIRN in order to help the nationwide/global effort on combatting antimicrobial resistance in veterinary settings.