7T functional MRS of metabolite variations during working memory in subjects with post-traumatic stress disorder

为了响应NOT-MH-21-120“COVID-19影响NIMH研究的行政补充”， 申请寻求对主要研究者的额外支持，以完成指导研究的目标 科学家发展奖编号K 01 MH 115272。该研究项目的目标是检查神经 这是导致创伤后应激障碍（PTSD）患者工作记忆缺陷的机制。 本项目结合7 T静息态磁共振波谱（MRS）、先进的动态功能磁共振成像（DFM）技术， 磁共振波谱（fMRS）和功能性磁共振成像（fMRI），以探讨 工作记忆中的神经化学和大脑激活。具体目标是（1）衡量 有和没有PTSD的人背外侧前额叶皮层（DLPFC）中的谷氨酸和其他代谢物 （2）研究静息态谷氨酸水平与功能性脑损伤的关系。 使用MRS和fMRI在有和没有PTSD的人中进行n-back工作记忆任务期间的激活;以及 (3)在PTSD患者和非PTSD患者的工作记忆中表征动态谷氨酸变化 n-back任务期间的fMRS。PI在实现这些目标方面取得了重大进展。然而，在这方面， COVID-19大流行导致招募和数据收集的重大延迟。在本申请中，我们 为PI请求补充支持，以弥补因COVID-19造成的时间损失，完成 人的目标，实现项目的目标。

In response to NOT-MH-21-120 “Administrative Supplements for COVID-19 Impacted NIMH Research,” this application seeks additional support for the principal investigator to complete the aims of the Mentored Research Scientist Development Award number K01MH115272. The goal of the research project is to examine the neural mechanisms that contribute to working memory deficits in people with posttraumatic stress disorder (PTSD). This project combines 7T resting-state magnetic resonance spectroscopy (MRS), advanced dynamic functional MRS (fMRS), and functional magnetic resonance imaging (fMRI) to investigate the relationship between neurochemistry and brain activation during working memory in PTSD. The specific aims are to (1) measure glutamate and other metabolites in the dorsolateral prefrontal cortex (DLPFC) of people with and without PTSD using resting-state MRS; (2) investigate the relationship between resting-state levels of glutamate and functional activation during the n-back working memory task in people with and without PTSD using MRS and fMRI; and (3) characterize dynamic glutamate changes during working memory in people with and without PTSD using fMRS during the n-back task. The PI has made substantial progress towards completing these aims. However, the COVID-19 pandemic has caused significant delays in recruitment and data collection. In this application, we request supplemental support for the PI to offset the loss of time due to COVID-19, complete enrollment of human subjects, and fulfill the aims of the project.