Pacific Northwest Center for Cryo-EM - Screening supplement

项目总结(来自原始申请) 低温电子显微镜(Cryo-EM)使生物医学科学中的成像技术发生了革命性的变化，从近原子 利用单粒子低温电子显微镜对单个生物分子的分辨率可视化 用冷冻电子断层扫描(CRYO-ET)原位检测大分子络合物。这场革命一直是 很大程度上是由计算机软件中用于收集低温电磁图像的探测器的进步推动的 用于图像分析和标本重建以及显微镜本身的程序。 因为支撑低温EM的技术既昂贵，又在短时间内戏剧性地进步 在一段时间内，许多研究小组可能受益于在他们的生物医学研究中使用低温EM 既没有获得低温电磁技术的机会，也没有接受过使用该技术的培训。要解决访问 为了低温电磁技术和教育，我们将创建太平洋西北低温电磁中心。PNCC将 是俄勒冈健康与科学大学(OHSU)和太平洋西北地区的合作项目 国家实验室(PNNL)。通过利用OHSU在高分辨率成像方面的优势，生物 结合PNNL在电子领域的长期经验，在低温EM中进行样品制备和教育 显微镜、高性能计算和强大的用户设施操作，我们将赋予PNCC 在单粒子低温EM和低温ET方面的杰出能力，以及与彻底和完整的 低温电磁教育或交叉培训计划。此外，由于PNCC已经建立了合作 凭借低温电磁技术以及计算机硬件和软件方面的领导者，PNCC将走在前列 技术能力。此外，OHSU参加了由 显微镜制造商FEI将支持PNCC的交叉培训计划。OHSU和PNNL都将 在PNCC进行重大投资。OHSU将为仪器提供一个低振动空间，它将 向该中心捐赠一台新显微镜，OHSU将提供1900万美元的额外资金用于太空使用 翻新、添置一台显微镜和中心工作人员的支助。与此同时，PNNL已经 承诺访问他们的高性能计算机中心、用户程序和高分辨率 电子显微镜。通过将两个优势互补的主要研究机构聚集在一起， 连同明智地选择的合作和主要的额外支持来源，我们将创建一个国家- 最先进的、以用户为中心的生物分子和大分子高分辨率成像中心 用于组装和教育在低温EM领域受过培训的下一代科学家。

PROJECT SUMMARY (from original application) Cryo-electron microscopy (cryo-EM) has revolutionized imaging in biomedical science, from near atomic resolution visualization of individual biological molecules by single particle cryo-EM to the definition of macromolecular complexes, in situ, using cryo-electron tomography (cryo-ET). This revolution has been drivenlargely by advances in the detectors employed to collect the cryo-EM images, in the computer software programs utilized in image analysis and specimen reconstruction, and in the microscopes themselves. Because the technology underpinning cryo-EM is both expensive and has dramatically advanced over a short period of time, many research groups that could benefit from incorporating cryo-EM in their biomedical studies neither have access to cryo-EM technology nor have the training to employ the technology. To address access to cryo-EM technology and education, we will create the Pacific Northwest Center for Cryo-EM. The PNCC will be a collaborative effort between the Oregon Health and Science University (OHSU) and the Pacific Northwest National Laboratory (PNNL). By harnessing the strengths of OHSU in high resolution imaging, biological sample preparation and education in cryo-EM with the long-standing experience of PNNL in electron microscopy, high performance computing and robust user facility operation, we will endow the PNCC with outstanding capabilities in single particle cryo-EM and cryo-ET, as well as with a thorough and complete program in cryo-EM education or cross-training. Moreover, because the PNCC has established collaborations with leaders in cryo-EM technology and in computer hardware and software, the PNCC will be at the forefront of technological capability. Furthermore, the participation of OHSU in a cryo-EM school co-sponsored by the microscope manufacturer, FEI, will bolster the cross-training program of the PNCC. Both OHSU and PNNL will make major investments in the PNCC. OHSU will provide a low vibration space for the instrumentation, it will donate a new microscope to the center and OHSU will provide $19M in additional funding for use in space renovation, acquisition of an additional microscope and support of center staff. At the same time, PNNL has pledged access to their high-performance computer centers, their user programs and their high-resolution electron microscopes. By bringing together two major research institutions with complementary strengths, together with judiciously chosen collaborations and major additional sources of support, we will create a state- of-the-art, user-focused center for the high-resolution imaging of biological molecules and macromolecular assemblies and for the education of the next generation of scientists trained in the field of cryo-EM.