Mouse Breeding and Procedure Core

摘要 小鼠繁育和程序核心(核心C)是项目顺利运行的关键 项目。核心C有两个主要功能。首先，Core C将提供并监督小鼠的繁殖和基因分型 服务。为了最大限度地提高质量，本项目的所有三个项目都将集中饲养MICE 控制和保持鼠标线条的一致性，并最大限度地提高效率，特别是对于这三种类型共享的线条 项目。核心成员将交配、出生、分离和断奶小鼠。核心将引导尾巴用于 纽约大学基因分型服务中心的基因分型服务。一旦进行了基因分型，Core C会将小鼠分配给进一步的 根据需要和获得的小鼠的基因/性别，进行繁殖或实验。核心C将 在6周龄前将所有研究用小鼠分配给三个项目。如个别项目所示，核心 C，通过核心A，实施随机化和战略，这对严谨和可重复性至关重要 以及对偏见的保护。第二，Core C将协调和执行鼠标程序。共享程序 在由Core C执行和监督的整个计划项目中，包括：主动脉移植手术， 致死照射和骨髓移植；脂肪组织移植；异种共生；葡萄糖和胰岛素 耐受性试验；影像研究(肝脏超声和磁共振成像以及地塞米松扫描)； 肝原代细胞包括肝细胞、枯否细胞和肝星状细胞的分离及分离 细胞外小泡的数量。核心C将在所有五年内为计划项目的所有三个项目提供服务。

Summary The Mouse Breeding and Procedure Core (Core C) is essential for the smooth functioning of the Program Project. Core C has two major functions. First, Core C will provide and oversee mouse breeding and genotyping services. Mice will be bred centrally for all three projects of the Program Project in order to maximize quality control and consistency of mouse lines, and to maximize efficiency, particularly for lines shared across the three Projects. Mice will be mated, born, separated, and weaned by the Core members. The Core will direct tails for genotyping to NYU Core for Genotyping Services. Once genotyped, Core C will allocate mice to either further breeding or to experimentation depending on the needs and the genotype/sexes of mice obtained. Core C will distribute all mice for studies to the three Projects by age 6 weeks. As indicated in the individual Projects, Core C, through Core A, implements randomization and strategies that will be essential for rigor and reproducibility and protection against bias. Second, Core C will coordinate and perform mouse procedures. Shared procedures across the Program Project to be performed and overseen by Core C include: aorta transplantation surgery, lethal irradiation and bone marrow transplantation; adipose tissue transplantation; parabiosis; glucose and insulin tolerance tests; imaging studies (liver ultrasound and magnetic resonance imaging and DEXA scanning); isolation of primary cells from the liver, including hepatocytes, Kupffer cells and hepatic stellate cells and isolation of extracellular vesicles. Core C will serve all three projects of the Program Project over all five years.