Biopolymers & Proteomics

生物聚合物与蛋白质组学核心：项目摘要/摘要 对蛋白质、多肽、小分子和代谢物进行灵敏和定量分析的能力至关重要。 了解细胞、组织和生物体的正常状态和疾病状态之间的差异。这样的分析 通常需要复杂的技术专业知识和最先进的仪器设备。生物聚合物与 蛋白质组学核心(B&P Core)为科赫研究所的研究人员提供了一系列的服务， 强调蛋白质/肽、蛋白质组学和质谱学服务，使合成和 生物材料的分析。这些是个别研究人员通常不会学习的技术，但它们是 对于现代癌症研究是必不可少的，因此核心提供了访问这些技术的途径。 在本供资期间，B&P核心增加了新的能力并修改了现有服务，包括： 利用机构来源的资金收购两个新的混合四极轨道MS仪器以扩大规模 蛋白质组学分析能力和提高灵敏度；利用机构来源的资金升级高效液相色谱至 能够改进复杂样品的分级并增加独立的高效液相色谱使用；以及扩展专业知识 用于高效液相分析和纯化(例如，小分子-药物结合物)。中心成员核心使用情况 强：84%的中心成员使用核心服务，占核心服务使用的74%，包括调查人员 来自所有三个研究项目。 在接下来的一段时间里，B&P Core将继续提供广泛的最先进的服务来支持 中心成员研究计划，并将在中心成员的背景下评估新兴能力 需求和兴趣。计划采取若干新举措，包括：扩大和加强 蛋白质组学在全球和定量蛋白质组分析、翻译后修饰检测方面的能力 和识别结合伙伴简档；以及改进了对质谱学相关研究人员的支持 信息学和复杂蛋白质组数据分析。B&P Core也致力于维护 仪器仪表处于领先地位，并将继续密切监测技术进步，特别是 关于LC-MS仪器的灵敏度、特异度、操作和速度。核心利用了 租赁和供应商寄售计划，以提供更频繁的仪器更新，允许核心 以较低的相对成本试验新的进步和技术。在即将到来的资助期，我们将进行一项 对中心成员在代谢组学能力方面的需求进行实际评估。为任何新的 设备采购将完全来自机构来源。这种共享资源是成功的关键 科赫研究所使命的一部分，为CCSG提供了非凡的价值。请求的CCSG年度预算 49比本期(48年)核心共同核心预算增加18.5%，反映增加 对蛋白质组学专业知识的支持。

Biopolymers & Proteomics Core: Project Summary/Abstract The ability to sensitively and quantitatively analyze proteins, peptides, small molecules and metabolites is critical to understanding differences between normal and disease states in cells, tissues and organisms. Such analyses typically require sophisticated technical expertise and state-of-the-art instrumentation. The Biopolymers & Proteomics Core (B&P Core) provides Koch Institute researchers with a diverse range of services, with an emphasis on protein/peptide, proteomic and mass spectrometry services, that enable both synthesis and analysis of biological materials. These are technologies that individual researchers usually do not learn, but are essential for modern cancer research, and as such the Core provides access to these techniques. In the current funding period, the B&P Core added new capabilities and modified existing services, including: acquisition of two new hybrid quadrupole-orbitrap MS instruments with funds from Institutional sources to expand proteomics analysis capacity and improve sensitivity; upgraded HPLC with funds from Institutional sources to enable improved fractionation of complex samples and added independent HPLC use; and expanded expertise in HPLC analysis and purification (e.g. small molecule-drug conjugates). Center Member Core use has been strong: 84% of Center Members use the Core, account for 74% of Core service use, and include investigators from all three Research Programs. In the upcoming period, the B&P Core will continue to offer a wide range of state-of-the-art services to support Center Member research programs, and will evaluate emerging capabilities in the context of Center Member needs and interests. A number of new initiatives are planned, including: expansion and enhancement of proteomics capabilities in global and quantitative proteomic analysis, detection of post-translational modifications and identification of binding partner profiling; and improved investigator support for mass spectrometry-related informatics and complex proteomic data analyses. The B&P Core is also committed to maintaining instrumentation at the leading edge, and will continue to closely monitor technological improvements, particularly with regard to LC-MS instrument sensitivity, specificity, operation, and speed. The Core has taken advantage of leasing and vendor consignment programs to provide more frequent instrumentation updates, allowing the Core to pilot new advances and technologies at lower relative cost. In the upcoming funding period, we will conduct a hands-on assessment of Center Member needs with respect to metabolomics capabilities. Funding for any new equipment acquisitions will be solely from Institutional sources. This Shared Resource is essential to the success of the Koch Institute mission and provides exceptional value to the CCSG. The requested CCSG budget for Year 49 is increased by 18.5% over the Core CCSG budget for the current period (Year 48), reflecting increased support for proteomics expertise.