Myosin 18 and its role in skeletal muscle

肌球蛋白18及其在骨骼肌中的作用：H.Lee Sweeney，P.I. 摘要： 本项目将研究一种非传统肌球蛋白--肌球蛋白XVIII(Myo18)的结构和功能。一 在脊椎动物中表达的两种形式Myo18中，Myo18B对于在脊椎动物中产生肌节至关重要 横纹肌。另一种形式Myo18A存在于所有细胞中，包括肌肉，它可能在其他细胞中发挥作用 需要Myo2细丝招募和组织的关键细胞过程，包括 横纹肌的肌节形成。导致Myo18B丢失的突变导致肌病 人类。虽然Myo18被描述为一种非运动的支架蛋白，它有助于组装 MyO2细丝，我们假设它实际上是一种受新形式调控的活性运动蛋白。仅限 通过了解这种马达的设计和功能，并利用这些见解来探索肌节的形成 在肌肉细胞中，我们是否会开始了解Myo18在横纹肌中所扮演的角色，并最终在 其他纸巾。

Myosin 18 and its role in skeletal muscle: H. Lee Sweeney, P.I. Abstract: This project will investigate the structure and function of an unconventional myosin, myosin XVIII (Myo18). One of the two forms Myo18 expressed in vertebrates, Myo18B, is critical for the generation of sarcomeres in striated muscle. The other form, Myo18A is found in all cells, including muscle, where it may function in other critical cellular processes that require Myo2 filament recruitment and organization, including the early stages of sarcomere formation in striated muscle. Mutations that result in the loss of Myo18B result in myopathies in humans. While Myo18 has been characterized as a non-motor, scaffolding protein, that aids in the assembly of Myo2 filaments, we postulate that it is in fact an active motor protein subject to a novel form of regulation. Only by understanding the design and function of this motor, and using those insights to probe sarcomere formation in muscle cells, will we begin to understand the roles that Myo18 plays in striated muscle and ultimately in other tissues.