Proteomics Core

OK-INBRE蛋白质组学核心-摘要 OK-INBRE蛋白质组学核心的目标是为俄克拉荷马州和其他地区的研究人员提供 符合IDEA资格的州可以获得最先进的仪器和先进质量的专业知识 光谱(MS)。核心配备了所需的仪器和专业知识 使发现蛋白质组学和有针对性的定量蛋白质组学实验可用于 俄克拉何马州的研究人员。发现蛋白质组学实验(特定目标1)将使用数据- 相关分析以获得高分辨率调查扫描以确定多肽质量 高精度碰撞诱导解离(CID)光谱测定氨基酸 序列。然后，数据库搜索识别蛋白质并找到翻译后 修改。有针对性的定量蛋白质组学实验(特定目标2)将使用 选择性检测的选择性反应监测(SRM)和平行反应监测(PRM) 并根据胰酶消化形成的多肽对蛋白质进行量化。以确保全州范围内 了解核心的能力和使用这些方法的途径(具体目标3)，OK- INBRE蛋白质组学核心实验室将，i)继续提供教育研讨会和开发 描述我们的方法并教育潜在用户的在线材料以及ii)提供凭单 支付与试验项目相关的费用，以提高OK-INBRE蛋白质组学的利用率 核心。最后，OK-INBRE蛋白质组学核心将继续作为目标 IDEA国家蛋白质组学资源的定量蛋白质组学组成部分，提供 获得先进的蛋白质组学方法并为核心董事、实习生和 来自所有符合IDEA资格的州的研究人员。

OK-INBRE Proteomics Core - Summary The goal of the OK-INBRE Proteomics Core is to provide researchers in Oklahoma and in all IDeA-eligible states access to state-of-the-art instrumentation and expertise for advanced mass spectrometry (MS). The core is equipped with the instrumentation and expertise required to make discovery proteomics and targeted quantitative proteomics experiments available to researchers in Oklahoma. The discovery proteomics experiments (Specific Aim 1) will use data- dependent analyses to acquire high resolution survey scans to determine peptide mass with high accuracy and collision induced dissociation (CID) spectra to determine the amino acid sequence. Database searches then identify the proteins and find posttranslational modifications. The targeted quantitative proteomics experiments (Specific Aim 2) will use selected reaction monitoring (SRM) and parallel reaction monitoring (PRM) to selectively detect and quantify proteins based on peptides formed by trypsin digestion. To ensure statewide awareness of the core's capabilities and access to these methods (Specific Aim 3), the OK- INBRE Proteomics Core laboratory will, i) continue to offer educational workshops and develop on-line materials to describe our methods and educate potential users and ii) provide vouchers to defray costs associated with pilot projects to enhance utilization of the OK-INBRE Proteomics Core. Finally, the OK-INBRE Proteomics Core will continue to function as the Targeted Quantitative Proteomics component of the IDeA National Resource for Proteomics that provides access to advanced proteomics methods and education for Core Directors, trainees, and researchers from all IDeA-eligible states.