CORE B- Neuropathology

项目概要（核心B） 在上一个资助周期中，神经病理学核心（Core B）建立了一个高效的脑组织 银行系统，极大地促进了研究项目在这个PPG的成功。在续约提案中， 核心B将继续其使命，提供高质量的第2-第3孕期，围产期和出生后早期人类 来自加州大学旧金山分校病理学系尸检服务处的脑组织， PPG。特别地，核心B将专注于制备用于单细胞RNA-seq（scRNA-seq）的组织、单核 RNA-seq（snRNA-seq）、RNAscope和超微结构分析，这将为研究人员和项目人员做好准备。 领导人在这个PPG表征细胞谱系的分子和细胞机制，在发展中国家， 人类的大脑核心B将开发最先进的分子标记，以促进发现和 正常人脑发育过程中神经元-神经胶质相互作用的特征。核心B将提供全部- 时间组织病理学服务，促进本PPG中研究项目的进展，以及 与内部和外部咨询委员会协调活动。从本质上讲， 神经病理学的核心是作为一个集中的设施，人类大脑组织和进步， 组织病理学技术可用于为所有调查人员提供支持和服务整合。的 核心主任将与项目领导人合作，就核心服务的使用作出决定。作为 作为PPG的积极成员，核心主任将参加有关行政和 科学问题，如研究方向，特定人体组织的要求，数据分析，合作 和数据的呈现。核心主任和联合主任与Alveraz-Buylla博士、Rowich博士协商， 博士花式，和朴博士，将评估的具体需求和成本效益，以最大限度地提高服务 每个研究项目的核心B。核心主任将确保提供最高质量控制 水平将通过使用我们的内部和外部咨询委员会来实现额外的质量控制。

Project Summary (Core B) In the previous funding cycle, the Neuropathology Core (Core B) established a highly effective brain tissue banking system that greatly facilitates the success of research projects in this PPG. In the renewal proposal, Core B will continue its mission to provide high quality 2nd – 3rd trimester, perinatal and early postnatal human brain tissues from the Autopsy Service in the Department of Pathology at UCSF for all the components of the PPG. In particular, Core B will focus on preparing tissues for single cell RNA-seq (scRNA-seq), single nucleus RNA-seq (snRNA-seq), RNAscope and ultrastructural analyses, which will prepare researchers and project leaders in this PPG to characterize the molecular and cellular mechanisms of cell lineages in the developing human brains. Core B will develop state-of-the-art molecular markers that will facilitate the discovery and characterizations of neuron-glia interactions during normal human brain development. Core B will provide full- time histopathology services that facilitate the progress among the research projects in this PPG as well as coordinate activities with the internal and external advisory committees. In essence, the aim of the Neuropathology Core is to function as a centralized facility where human brain tissues and advances in histopathology techniques can be utilized to provide support and integration of services for all investigators. The core director will work with the project leaders and make decisions regarding the use of core services. As an active member of the PPG, the core director will participate in weekly meetings regarding administrative and scientific matters such as research directions, requests for specific human tissues, data analyses, collaborations and presentations of data. The core director and co-director, in consultation with Dr. Alveraz-Buylla, Dr. Rowitch, Dr. Fancy, and Dr. Piao, will evaluate the specific needs and cost-effectiveness in order to maximize the service of Core B to each research project. The core director will ensure that quality control is provided at the highest level. Additional quality control will be achieved through the use of our internal and external advisory committees.