Prenatal Cigarette Smoke Exposure: Impact on Offspring Gut Bacterial Microbiome

摘要 这份促进健康相关研究多样性的研究补充材料是为了支持一名研究生 执行由R15奖2R15ES028440-02A1资助的研究，产前吸烟暴露：影响 子代肠道细菌微生物群。以赛亚·布尔恰加先生在多个方面都有资格获得该奖项：他是一位 来自农村、经济困难的理科代表不足的小规模(非洲裔)学生 背景谁是第一代大学生。他对一项生物医学研究表现出了兴趣 并在学术和技术上都表现出了实现这一职业目标的能力。这些研究 在父母奖中提出使用发育良好的烟草暴露小鼠模型 烟。我们已经展示了产前吸烟暴露(CSE)对新生儿体重、追赶生长、 以及小鼠母鼠及其后代的肠道细菌菌群，以及对后代表型的影响。 然而，我们不知道观察到的微生物组和后代表型的变化是否有联系。我们会 测量盲肠微生物群或饮食抗性淀粉的转移对这些措施的影响 模特。我们的中心假设是CSE诱导的肠道微生物群变化与后代的表型有关， 将在两个目标内进行测试： 目的1：利用断奶仔猪盲肠移植分离CSE诱导的微生物群和CSE本身 目的2：在青春期前和青春期后使用抗膳食淀粉增加短链脂肪酸 如果我们的中心假设得到支持，更多的研究将深入研究肠道-肝脏-脑轴的后果 肠道生物失调，以及调查潜在干预措施。Burciaga先生的研究培训将集中在 主要针对目标2，并将包括通过UofL毕业生参与专业发展活动 学校、参加科学会议、基金撰写和出版。

ABSTRACT This Research Supplement to Promote Diversity in Health-Related Research is to support a graduate student to perform research funded by R15 award 2R15ES028440-02A1, Prenatal Cigarette Smoke Exposure: Impact on Offspring Gut Bacterial Microbiome. Mr. Isaiah Burciaga is eligible for this award on multiple fronts: he is an underrepresented minoritized (Afro-Latino) student in science from a rural, economically disadvantaged background who is a first-generation college student. He has demonstrated interest in a biomedical research career and has exhibited ability both academically and technically to fulfill that career goal. The studies proposed within the parent award use a well-developed murine model of developmental exposure to tobacco smoke. We have shown effects of prenatal cigarette smoke exposure (CSE) on birth weight, catch-up growth, and gut bacterial microbiome in mouse dams and their offspring, as well as effects on offspring phenotype. However, we do not know if the observed changes in microbiome and offspring phenotype are linked. We will measure the effect of cecal transfer of microbiomes or dietary resistant starch on those same measures in this model. Our central hypothesis, that CSE-induced gut microbiome alterations are linked to offspring phenotype, will be tested within 2 aims: Aim 1: use cecal transfer in weanlings to separate effects of CSE-induced microbiome from CSE itself Aim 2: use dietary resistant starch to increase short-chain fatty acids before vs after puberty If our central hypothesis is supported, additional studies will delve into gut-liver-brain axis consequences of intestinal dysbiosis, as well as investigate potential interventions. Mr. Burciaga’s research training will focus primarily on Aim 2 and will include participation in professional development activities through the UofL Graduate School, participation in scientific conferences, grant writing, and publication.