NITROUS OXIDE CONSCIOUS SEDATION--ANXIOYSIS

一氧化二氮被广泛用于生产清醒镇静。 临床牙科。低浓度阿司匹林的临床应用 专门用于降低焦虑的一氧化二氮是牙科所特有的。 一氧化二氮也有显著的止痛剂和弱麻醉剂。 已被广泛研究的特性。然而，它的抗焦虑作用 尚未得到很好的调查和潜在的作用机制(S) (S)不为人知。最近的研究表明，一种明显的抗焦虑药物 在小鼠阶梯试验中，一氧化二氮的作用是明显的。 相对较新的焦虑范式。本申请拟试点 确定未来基础研究的可行性的实验 一氧化二氮抗焦虑作用的机制。这样做的目的是 建议的研究是研究2个已建立和验证的实验 范式(社交测验和防御性掩埋测验 大鼠)：(1)一氧化二氮的浓度依赖的抗焦虑作用 与氯氮卓酮(抗焦虑标准)和吗啡(AN)的比较 止痛标准)；(2)这3种药物的药效敏感性 相互作用的挑战(一氧化二氮、氯氮卓酮和吗啡) 使用阿片类药物和苯二氮卓类受体阻滞剂。带到了一个成功的 结语，本研究的提出将使更多更深入的研究成为可能。 关于抗焦虑作用的复杂研究 对一氧化二氮的影响及其作用机理的阐明(S)。《长河》 本研究的学期目标是继续系统地分析 氧化亚氮的药理性质。这类研究的发现 应促进我们对一氧化二氮的药理作用和 有助于一氧化二氮更有效或更明智的临床应用 在牙科和医学方面。

Nitrous oxide is widely used for production of conscious sedation in clinical dentistry. This clinical application of low concentrations of nitrous oxide specifically for reduction of anxiety is unique to dentistry. Nitrous oxide also possesses prominent analgesic and weak anesthetic properties that have been widely studied. However, its anxiolytic effect has not been well investigate and the underlying mechanism(s) of action remain(s) unknown. Recent research suggests that an apparent anxiolytic effect of nitrous oxide is demonstrable in the mouse staircase test, a relatively new anxiety paradigm. This application proposes pilot experiments to determine the feasibility of future basic research into the mechanism of anxiolytic effect of nitrous oxide. The goals of this proposed research are to study 2 established and validated experimental paradigms (the social interaction test and the defensive burying test in rats): (1) a concentration-dependent anxiolytic effect of nitrous oxide and comparison with chlordiazepoxide (an anxiolytic standard) and morphine (an analgesic standard); and (2) sensitivity of the effects of these 3 drug challenges (nitrous oxide, chlordiazepoxide and morphine) to interaction with opioid and benzodiazepine receptor blockers. Carried to a successful conclusion, this proposed research will make possible further and more sophisticated investigations for characterization of the anxiolytic effect of nitrous oxide and elucidation of its mechanism(s) of action. The long term objective of this research is to continue a systematic analysis of the pharmacological properties of nitrous oxide. The findings of such research should advance our understanding of the pharmacology of nitrous oxide and contribute to more efficacious or judicious clinical use of nitrous oxide in dentistry and medicine.