TOPICAL IN-VIVO ELECTRON SPIN RESONANCE

摘要：（改编自摘要）。 潜在的实用性和 局部体内ESR的重要性被引用为以下独特的 它与其他技术的区别： a）外来顺磁性物质（自旋标记）的体内检测 靶向生物系统中的特定位点。 B）体内监测 使用自旋标记在微观水平上监测结构顺序， （膜、蛋白质、细胞壁等）c）直接体内检测游离的 自由基物质（天然存在的或“捕获”的瞬态产物） 自由基反应）。 本研究资源的具体目标 发展更新建议包括： 1. 低场体内局部ESR的进一步发展和优化 光谱仪，包括低GHz范围内的微波谐振器 （500-1000 MHz）;评估可行性、灵敏度、功率损耗和 低场局部ESR系统的规格。 2. 在体内监测自旋标记分布和代谢， 动物： a.氮氧代谢和排泄体内药代动力学 B.用自旋标记代谢监测肿瘤生长特性 C.猪全脑缺血心肌自旋捕集研究 再灌注模型; D.大脑中的氮氧化物监测

DESCRIPTION: (Adapted from the Abstract). The potential usefulness and importance of topical in-vivo ESR are cited as the following unique properties which distinguish it from other techniques: a) In-vivo detection of extrinsic paramagnetic species (spin labels) targeted to specific sites in a biological system. b) In vivo monitoring using spin labels which monitor structural order at the microscopic level (membranes, proteins, cell walls, etc.) c) Direct in-vivo detection of free radical species (naturally occurring or "trapped" products from transient free radical reactions). The specific aims of this Research Resource development renewal proposal are: 1. Further development and optimization of low field in-vivo topical ESR spectrometers, including microwave resonators in the low GHz range (500-1000 MHz); assessment of feasibility, sensitivity, power losses, and specifications of a low field topical ESR system. 2. In-vivo monitoring of spin label distribution and metabolism in animals: a. in-vivo pharmacokinetics of nitroxide metabolism and excretion b. monitoring tumour growth characteristics by spin label metabolism c. spin trapping studies of a swine global myocardial ischemia reperfusion model; d. nitroxide monitoring in the brain.