Production And Characterization Of HIV-1 Solid Phase Proteoliposomes

单体HIV-1 gp 120外被膜糖蛋白未能有效地引发中和抗体。因此，我们尝试将三聚体天然g120结构重建为含有HIV包膜糖蛋白（EnvPL）的固相蛋白脂质体（PL）。这项研究的特点是天然的三聚体结构的糖蛋白在脂质双层环境中，并将测试它们作为免疫原。目前，我们正在测试来自更具抗性的主要分离株的新版本，交叉进化枝EnvPL版本，以及检查与Env-PL组合的选定佐剂。 我们还将在固相脂质体中重建7-跨膜HIV-1共受体分子（CCR 5或CXCR 4），并评估其结合趋化因子或HIV-1 gp 120-CD 4复合物的功能。我们还将开发Biacore测定法和脂质重建ELISA（L-ELISA）来测试配体与辅助受体分子的结合。

Monomeric HIV-1 gp120 exterior envelope glycoproteins have failed to efficiently elicit neutralizing antibodies. We have therefore attempted to reconstitute the trimeric native g120 structure as solid phase proteoliposomes (PLs) containing HIV envelope glycoproteins (EnvPLs). This study characterizes the native trimeric structure of the glycoproteins in a lipid bilayer environment and will test them as immunogens. Currently were are testing new versions from a more resistant primary isolate, cross-clade EnvPL versions as well as examining selected adjuvants in combination with the Env-PLs. We will also reconstitute the 7-membrane spanning HIV-1 co-receptor molecules (CCR5 or CXCR4) in solid phase liposomes and assess their function to bind chemokine or HIV-1 gp120-CD4 complexes. We will also develop both a Biacore assay and a lipid-reconsituted ELISA (L-ELISA) to test binding of ligands to the co-receptor molecules.