System for multidimensional analysis of growth and migration of neurons and neuro

描述（由申请人提供）：我们提出了Orion工作站，这是一种创新的下一代显微镜系统，为神经突生长动力学和神经元前体迁移的全自动定量变化分析提供了分析工具。构成该软件基础的算法提供了在单个神经突和细胞中观察到的变化的信息，并产生了总结观察到的变化的结果。然而，结果将超出每个时间点所有神经元结构的总结量化，因为分析工具用于匹配相邻图像之间的单个对象，从而增加了研究间的可靠性和延时实验的可重复性。与目前可用的技术相比，Orion工作站将能够对体外分离细胞培养中神经突生长的动态（即随着时间的推移而变化）和原位培养中神经元前体的迁移进行复杂而详细的分析。公共卫生相关性：提出的Orion工作站将为基础神经科学以及神经毒理学和药理学研究和开发开辟新的视野，通过提供一个强大而有效的多维过程来检测和分析神经突的生长和神经元前体的迁移。该项目的总体效果将允许以经济、系统的方式收集高度相关的、多方面的数据，描述培养系统内的多个细胞。

DESCRIPTION (provided by applicant): We propose the Orion workstation, an innovative, next generation microscope system that provides analysis tools for fully-automated quantitative change analysis in neurite outgrowth dynamics and neuronal precursor migration. The algorithms that will form the basis of this software provide information about changes seen in individual neurites and cells, yielding results that summarize observed changes. The results will go beyond summary quantifications of all neuronal structures at each time point, however, because analysis tools are used to match individual objects between adjacent images thereby increasing inter-study reliability and reproducibility of time-lapse experiments. The proposed Orion workstation will enable sophisticated and detailed analysis of the dynamics of neurite outgrowth (i.e., as they change over time) in dissociated cell cultures in vitro and migration of neuronal precursors in slic cultures in situ compared to currently available technology. PUBLIC HEALTH RELEVANCE: The proposed Orion workstation will open new horizons in basic neuroscience as well as in neurotoxicology and pharmacological research and development, by providing a powerful and efficient multi-dimensional process to detect and analyze neurite outgrowth and migration of neuronal precursors. The overall effect of this project will permit the collection of highly relevant, multifaceted data describing multiple cells within a culture system in an economical, systematic manner.