System for multidimensional analysis of growth and migration of neurons and neuro

描述（由申请人提供）：我们提出了Orion工作站，这是一种创新的下一代显微镜系统，可为神经突生长动力学和神经元前体迁移的全自动定量变化分析提供分析工具。构成该软件基础的算法提供了有关单个神经突和细胞变化的信息，产生了总结观察到的变化的结果。然而，结果将超出每个时间点所有神经元结构的总结量化，因为分析工具用于匹配相邻图像之间的单个对象，从而增加了延时实验的研究间可靠性和再现性。所提出的Orion工作站将能够对神经突生长的动力学进行复杂和详细的分析（即，因为它们随时间变化），以及与目前可用的技术相比，神经元前体在原位SLIC培养物中的迁移。公共卫生相关性：Orion工作站将为基础神经科学以及神经毒理学和药理学研究和开发开辟新的视野，提供强大而有效的多维过程来检测和分析神经元前体的神经突生长和迁移。该项目的总体效果将允许以经济、系统的方式收集描述培养系统内多个细胞的高度相关、多方面的数据。

DESCRIPTION (provided by applicant): We propose the Orion workstation, an innovative, next generation microscope system that provides analysis tools for fully-automated quantitative change analysis in neurite outgrowth dynamics and neuronal precursor migration. The algorithms that will form the basis of this software provide information about changes seen in individual neurites and cells, yielding results that summarize observed changes. The results will go beyond summary quantifications of all neuronal structures at each time point, however, because analysis tools are used to match individual objects between adjacent images thereby increasing inter-study reliability and reproducibility of time-lapse experiments. The proposed Orion workstation will enable sophisticated and detailed analysis of the dynamics of neurite outgrowth (i.e., as they change over time) in dissociated cell cultures in vitro and migration of neuronal precursors in slic cultures in situ compared to currently available technology. PUBLIC HEALTH RELEVANCE: The proposed Orion workstation will open new horizons in basic neuroscience as well as in neurotoxicology and pharmacological research and development, by providing a powerful and efficient multi-dimensional process to detect and analyze neurite outgrowth and migration of neuronal precursors. The overall effect of this project will permit the collection of highly relevant, multifaceted data describing multiple cells within a culture system in an economical, systematic manner.