Human Embryonic Stem Cell Core Laboratory

hESC核心自2003年以来一直存在，已经获得并部分表征了21个中的14个。 NIH批准的hESC系。在此期间，它已经建立了作为hESC有效资源的声誉 在高度合作的科学界的专业知识。核心将继续协助PPG， UW/FHCRC调查员，表征和存储ESC线，使线和衍生物可用于 研究人员在MTA的参数范围内，培训研究人员，设计新的hESC培养方法， 并表征未分化和分化的hESC中的微（微）RNA。 在具体目标1，我们将支持hESC研究在UW/FHCRC通过获取和表征， 剩下的七个NIH批准的hESC系，我们将建立一个ES细胞动物园，我们将继续提供 向UW/FHCRC调查人员提供咨询和协助。在具体目标2中，我们将开发第二代 hESC培养条件，建立在我们最初的观察基础上，即4碳脂肪酸，丁酸， 在没有条件培养基的情况下支持hESC自我更新。我们还将研究 在丁酸盐存在下的各种基质。在具体目标3中，我们将描述所有NIH批准的 hESC系的RNA和microRNA谱，旨在揭示microRNA在hESC中的功能的研究。 人胚胎干细胞

The hESC Core has been in existence since 2003, and has acquired and partially characterized 14 of the 21 NIH approved hESC lines. During this time it has established a reputation as an effective resource for hESC expertise within a highly collaborative scientific community. The Core will continue to assist PPG and UW/FHCRC investigators,characterize and store ESC lines, make the lines and derivatives available to investigators within the parameters of the MTA's, train investigators,devise new methods of hESC culture and characterize mico(mi)RNAsin undifferentiated and differentiated hESCs. In Specific Aim 1 we will support hESC research at UW/FHCRC by acquiring and characterizing the remaining seven NIH approved hESC lines, we will build an ES cell zoo,and we will continue to provide advice and assistance to UW/FHCRC investigators. In Specific Aim 2 we will develop second generation hESC culture conditions, building upon our initial observation that the 4 carbon fatty acid, butyrate,can support hESC self renewal in the absence of conditioned medium. We will also examine the effects of various matrices in the presence of butyrate. In Specific Aim 3 we will characterize all the NIH-Approved hESC lines for RNA and micro RNA Profiles, studies designed to reveal the function of microRNAs in hESCs.