Hyperlens Imaging of Synaptic Vesicle Dynamics

我们正在进行一项成像计划，以建立一个超透镜纳米镜，使用新的先进的超材料超透镜技术，以实现上级的时间和空间分辨率，并调查膜动力学在脑突触传递和细胞内吞/胞吐一般。超透镜由专门设计和微制造的结构元件组成，其可以以亚衍射极限空间分辨率放大近场图像，并在真实的时间中在远场投射高分辨率图像（Yao等人，2008，Science，321：930; Liu等人，2008，Science，321：930）。等人，2007，Science，315：1686; Fang，et.等人，2005，Science，308：534-7）。 这种形式的光学纳米镜与其他侵入性和缓慢扫描、结构化照明或基于单探针定位的成像技术（诸如EM、STED（Hell，2007，Science，316：1153-8）和STORM/PALM（Huang等人，2008，Science，319：810-813; Bates，et.等人，2007，Science 317，1749-53; Hess等人，2007，PNAS 104，17370-5; Betzig等人，2007，Science 317，1749 - 53; Hess等人，2007，PNAS 104，17370-5; Betzig等人，2007，PNAS 104，17370-5。等人，2006，Science 313：1642）。 超透镜相关技术在X教授的团队的领导下正在迅速发展。张（加州大学伯克利分校）和孙成教授（现为西北大学的校外合作者）。 我们认为，超透镜成像应适用于调查重要的分子和细胞事件，成像分辨率和成像速度都是必不可少的。这个合作团队已经从trans-NIH成像计划中获得了设备资助，现在正在NIH开发必要的成像设置。我们正在努力扩展这项技术，并研究胞吐和胞吞过程中的膜动力学，其中囊泡融合和分裂是基础生物学和医学的基础。

We are undertaking an imaging initiative to build a hyperlens nanoscope, using newly advanced meta-material hyperlens technology, to achieve superior temporal and spatial resolution and to investigate membrane dynamics during brain synaptic transmission and cellular endocytosis/exocytosis in general. The hyperlens consists of specially designed and micro-fabricated structure elements that can magnify near-field images at sub-diffraction-limited spatial resolution and project the high resolution image at far-field in real time (Yao, et al, 2008, Science, 321:930; Liu, et. al, 2007, Science, 315:1686; Fang, et. al, 2005, Science, 308:534-7). This form of optical nanoscope possesses a unique advantage of both near real time optical imaging (at video rate) and nanometric spatial resolution, in compare with other invasive and slow scanning, structured-illumination, or single-probe localization based imaging techniques such as EM, STED (Hell, 2007, Science, 316: 1153-8), and STORM/PALM (Huang, et al., 2008, Science, 319:810-813; Bates, et. al, 2007, Science 317, 1749-53; Hess, et al, 2007, PNAS 104, 17370-5; Betzig, et. al, 2006, Science 313:1642). Hyperlens-related technology is under rapid development led by the groups of Prof. X. Zhang (UC Berkeley) and Prof. Cheng Sun (the extramural collaborator now at Northwestern University). We believe that hyperlens imaging should be applied to investigate important molecular and cellular events where both imaging resolution and imaging speed are essential. This collaboration team has won an equipment grant from the trans-NIH imaging initiative and is now developing the necessary imaging setup at NIH. We are working to extend the technique and to investigate membrane dynamics during exocytoses and endocytosis where vesicle fusion and fission underpin both fundamental biology and medicine.