Acquisition of a High Resolution Orbitrap Mass Spectrometer for Analysis of Prote

描述（由申请人提供）：在复杂生物样品中鉴定蛋白质翻译后修饰（PTMs）的能力在现代生物医学研究中越来越重要。该提案要求为购买配备纳米流液相色谱和电子转移解离（ETD）系统的ThermoFisher LTQ Orbitrap XL质谱仪提供资金。这将是具有挑战性的PTM分析的蛋白质组学平台的核心部分。该仪器将为蛋白质鉴定提供超高分辨率和高质量精度，并具有多级串联质谱分析和新型解离分析方法的独特功能。在普渡大学西拉斐特校区60英里半径范围内，没有类似的仪器为NIH的10个主要用户和7个次要用户提供服务。在普渡大学校园增加LTQ-Orbitrap质谱仪将对生物医学研究界进行前沿基础和应用研究的能力产生重大影响。这项工作将补充以研究者为基础的仪器开发和应用研究，并提供一个平台，及时将最先进的PTM制图方法转移到更广泛的生物学家群体中。国内学术单位，普渡癌症中心和宾德利生物科学中心，将与pi合作，确保适当的管理和高质量数据生产的最佳条件。该仪器将提供PTM分析平台的重要组成部分，并作为普渡大学癌症中心几个成员的主要分析工具。

DESCRIPTION (provided by applicant): The ability to identify protein post-translational modifications (PTMs) in complex biological samples is increasingly important in modern biomedical research. This proposal requests funds for the purchase of a ThermoFisher LTQ Orbitrap XL Mass Spectrometer equipped with nanoflow liquid chromatography and electron transfer dissociation (ETD) system. This will be the center piece for the proteomics platform for challenging PTM analyses. This instrument will provide ultra high resolution and high mass accuracy for confident protein identification and have unique features for multi-stage tandem mass spectrometry and novel dissociation methods for PTM analysis. There are no similar instruments to serve the NIH user group of 10 major and 7 minor users within a 60-mile radius of the Purdue University West Lafayette campus. The addition of the LTQ-Orbitrap mass spectrometer on the Purdue campus will have significant impact on the capacity of the biomedical research community to conduct leading edge basic and applied research. This effort will complement investigator-based research on instrument development and applications and provide a platform for timely transfer of state-of-art approaches in PTM mapping to a broader group of biologists. The home academic units, the Purdue Cancer Center and the Bindley Bioscience Center, will work cooperatively with the PIs to ensure the proper management and optimum conditions for high quality data production. The instrument will provide a vital component of the PTM analysis platform and serve as a major analysis tool for several members of the Purdue Cancer Center.