Console upgrades for biological NMR spectrometers

描述（由申请人提供）：申请资金用于更换位于范德比尔特大学结构生物学中心（VCSB）的配备冷冻探针的600 MHz和800 MHz NMR光谱仪的旧NMR控制台，并使用最先进的控制台。目前用于VCSB溶液NMR光谱仪的布鲁克DRX控制台已有8年历史，技术上已经过时，并开始频繁出现故障。申请的资金将与机构承诺合并，用于将2个最关键的老化控制台更换为Bruker Avance III控制台。除了提高核磁共振设备的可靠性外，新的控制台及其集成软件预计将比目前过时的系统性能高得多，并具有更大的技术能力。新控制台所需的仪器每天由专门的NMR设施经理Markus Voehler博士进行监督，他是一位有着20多年生物分子系统工作经验的资深NMR光谱学家。新的控制台是至关重要的成功，美国国立卫生研究院资助的生物分子研究的一组四个主要用户（C。Sanders，W. Chazin，M. P. Stone，A. Krezel），他们都是生物分子NMR方面的专家，以及次要用户（在本申请中由B表示）。艾希曼，B。哈德逊，and J. Meiler）.用户组的工作包括从NMR角度来看要求极高的研究，包括对膜内蛋白质、大的可溶性蛋白质和聚集分子量在100 kD范围内的蛋白质-蛋白质复合物的溶液NMR研究。许多项目涉及与人类疾病直接相关的生物分子问题。公共卫生相关性：核磁共振（NMR）光谱是一种强大的技术，用于研究生物分子的三维结构，运动和相互作用。范德比尔特大学的一些NIH支持的研究人员使用NMR来研究与人类健康和疾病直接和间接相关的生物分子。该申请要求提供所需的资金，以完全最先进的设备取代过时和越来越不可靠的NMR设备，这对于与该应用相关的研究人员的生物和生物医学研究计划的持续成功以及越来越多的用户社区的持续成功至关重要。

DESCRIPTION (provided by applicant): Funds are requested for the replacement of old NMR consoles for cryoprobe-equipped 600 MHz and 800 MHz NMR spectrometers located in the Vanderbilt University Center for Structural Biology (VCSB) with state-of-the-art consoles. The current Bruker DRX consoles for the solution NMR spectrometers of the VCSB are now 8 years old and are technically out of date and beginning to break down on a frequent basis. Funds are requested that will be pooled with institutional commitments to replace the 2 most critical of the aging consoles with Bruker Avance III consoles. In addition to enhancing the reliability of the NMR equipment, the new consoles and their integrated software are expected to perform at a much higher level than the current out-of-date systems and also to have expanded technical capabilities. The instrumentation for which the new consoles are sought is overseen on a daily basis by a dedicated NMR facilities manager, Dr. Markus Voehler, an accomplished NMR spectroscopist with over 20 years of experience working on biomolecular systems. The new consoles are critical to the success of the NIH-funded biomolecular research of both a group of four major users (C. Sanders, W. Chazin, M. P. Stone, A. Krezel), all of whom are experts in biomolecular NMR, and of minor users (represented in this application by B. Eichman, B. Hudson, and J. Meiler). Included in the work of the user groups are studies that are extremely demanding from an NMR standpoint, including solution NMR studies of integral membrane proteins, large soluble proteins and protein-protein complexes that have aggregate molecular weights in the range of 100 kD. A number of the projects involve biomolecular problems of direct relevance to human disease. PUBLIC HEALTH RELEVANCE: Nuclear magnetic resonance (NMR) spectroscopy is a powerful technique that is used to study the three-dimensional structures, motions, and interactions of biological molecules. NMR is used by a number of NIH-supported investigators at Vanderbilt University to study biomolecules that are of both direct and indirect relevance to human health and disease. The application requests the funds required to replace out-of-date and increasingly-unreliable NMR equipment with fully state-of-the-art equipment, as is critical to the continuing success of the biological and biomedical research programs of the investigators associated with this application and a growing community of additional users who are just realizing the power of NMR.