Acquisition of a Hybrid LC/MS/MS to Enhance Research in Nevada

描述（由申请人提供）：申请资金为内华达州蛋白质组学中心（NPC）购买QSTAR Elite混合LC-MS/MS质谱仪和克里思纳米多维液相色谱（MDLC）系统。所要求的仪器是一种用于在线液相色谱-质谱（LC-MS/MS）的电喷雾接口仪器，具有高灵敏度、出色的质量准确度、质量分辨率、动态范围和检测能力。购买这种用于翻译后修饰（PTM）位点定位、蛋白质-蛋白质相互作用和蛋白质鉴定研究的最先进的LC-MS/MS仪器对以下方面至关重要：1）当前研究计划的持续成功，这些研究计划解决与各种主题相关的基本问题，包括植物中的非生物胁迫、钙信号、平滑肌结构和氯离子通道的调节; 2）大学吸引新的尖端研究项目的能力，以及3）提供质谱技术领域的研究生培训。NPC是全州范围内的核心设施，服务于整个内华达州高等教育系统（NSHE），是内华达州内两大大学校园中唯一提供蛋白质组学分析的设施。该中心为内华达大学里诺分校（UNR）、内华达大学拉斯维加斯分校（UNLV）和内华达州癌症研究所（NCI）的大量资金充足的生物医学、生物化学和生物技术研究项目提供蛋白质组学和质谱分析服务。在过去的两年中，该中心分析了42名NSHE教职员工，18名外部学术教师用户和7名商业用户提交的3.7万多个样本。基于研究小组的数量，对LC-MS/MS服务的需求不断增加，以及MS技术对生物医学和生物学研究的重要性日益增加，很明显，新仪器的容量增加和新功能对于UNR和其他内华达州机构的研究计划的持续竞争力和成功至关重要。总的来说，拟议的文书将促进和允许扩大目前筹集超过5 600万美元的研究和培训项目。公共卫生相关性：拟议的仪器将促进国家卫生研究院支持的研究在内华达州高等教育系统（NSHE）。这项研究包括：1）平滑肌生理学研究糖尿病胃轻瘫，溃疡性结肠炎，心脏病和肌肉营养不良2）神经元生长和神经元再现性3）癌症研究检查肿瘤细胞死亡和衰老4）干细胞可塑性。

DESCRIPTION (provided by applicant): Funds are requested to purchase a QSTAR Elite Hybrid LC-MS/MS mass spectrometer and Tempo nano multi-dimensional liquid chromatography (MDLC) system for the Nevada Proteomics Center (NPC). The instrument being requested is an electrospray- interfaced instrument for on-line liquid chromatography-mass spectrometry (LC-MS/MS) offering a combination of high sensitivity, exceptional mass accuracy, mass resolution, dynamic range, and detection power. Purchase of this state-of-the-art LC-MS/MS instrumentation for posttranslational modification (PTM) site mapping, protein-protein interactions, and protein identification studies is critically important to the: 1) continued success of current research programs that address fundamental questions related to diverse topics, including abiotic stress in plants, calcium signaling, smooth muscle structure, and regulation of chloride channels; 2) the ability of the university to attract new cutting-edge research programs, and 3) provide graduate students training in the area of Mass Spec technology. The NPC is a statewide core facility serving the entire Nevada System of Higher Education (NSHE) and is the only facility on either of the two major university campuses within Nevada offering proteomic analysis. The center provides proteomic and mass-spectrometry services to a large number of well-funded biomedical, biochemistry, and biotechnology oriented research programs at the University of Nevada-Reno (UNR), - Las Vegas (UNLV), and the Nevada Cancer Institute (NCI). In the past two years the Center has analyzed more than 37 thousand samples submitted by 42 NSHE faculty members, 18 external academic faculty users and 7 commercial users. Based on the number of research groups, the ever increasing demand for LC-MS/MS services, and the increasing importance of MS technologies to biomedical and biological research, it is clear that the increased capacity and new capabilities of the new instrument is critically important to the continued competitiveness and success of research programs at UNR and other Nevada institutions. In aggregate, the proposed instrument will facilitate and allow expansion of research and training projects currently garnering in excess of $56 million. PUBLIC HEALTH RELEVANCE: The proposed instrumentation will facilitate NIH supported research within the Nevada System of Higher Education (NSHE). This research includes: 1) Smooth muscle physiology looking at diabetic gastroparesis, ulcerative colitis, Cardiac disease and Muscular Dystrophy 2) Neuronal growth and Neuron reproducibility 3) Cancer Research examining Tumor cell death and Senescence 4) Stem cell plasticity.