Acquisition of a 600-MHz Nuclear Magnetic Resonance Spectrometer

描述（由申请人提供）：本申请申请资金用于购买600 mhz核磁共振（NMR）光谱仪，以支持美国国立卫生研究院资助的爱荷华大学的研究项目，涵盖广泛的医学相关主题。该仪器结合了最新的磁体技术，以及在内部通信逻辑、射频信号生成和信号检测方面的增强，能够比前几代仪器更有效地获取高质量的数据。提议的四通道系统配置，以及关键附件，包括一个逆三共振1毫米探针，将使该仪器能够执行几乎任何最先进的小分子或生物分子研究的核磁共振实验。所要求的仪器旨在取代爱荷华大学中央核磁共振设施中老化的第一代高场600 mhz光谱仪，该光谱仪近20年来一直被大量共享使用，以支持许多美国国立卫生研究院资助的项目。拟议的文书将由经验丰富的工作人员维护，并主要由个人用户操作。校园内有30多个研究小组使用中央核磁共振设施，其中80%以上涉及生物医学研究。所建议的仪器已被选中，以满足当前和预期的未来需要的主要美国国立卫生研究院赞助用户，以及其他生物医学研究人员在校园。本申请中描述的来自11个主要用户组的12个nih支持的项目为购买拟议的光谱仪提供了理由。这些项目包括：真菌生物活性天然产物的结构研究；持久性有机污染物的合成、表征及毒理学研究新型三聚糖聚糖低聚糖组分的分离与结构分析生物电子传递系统中辅助因子的同位素标记分析多巴胺重要代谢产物氨基酸和肽偶联物分析对抗抗菌素耐药性的分子方法；潜在抗癌天然产物及相关化合物的合成与结构表征酶和信号结构域的结构与动力学分析；nod样受体途径选择和信号传导的分子基础；以及钙调蛋白结构域功能的分子基础。这些项目的主题要么是针对癌症、结核病、肌肉萎缩症或帕金森病的新疗法的发现，要么是针对扩展其他医学相关领域的基础知识。获得提议的仪器的最先进的核磁共振能力将有利于所有这些努力。公共卫生相关性：所申请的光谱仪将用于支持针对癌症、结核病、疟疾和其他传染病的新疗法的发现和开发的研究。致力于提高我们对肌肉萎缩症、帕金森氏病和哮喘以及环境污染物毒理学的分子水平理解的项目也将受益于该仪器的使用。

DESCRIPTION (provided by applicant): This application requests funding to enable the purchase of a 600-MHz nuclear magnetic resonance (NMR) spectrometer to support NIH-sponsored research projects spanning a wide range of medically relevant topics at the University of Iowa. The proposed instrument incorporates the latest magnet technology, as well as enhancements in internal communication logic, radiofrequency signal generation, and signal detection that enable more efficient acquisition of substantially higher quality data than earlier generations of instruments. The proposed four-channel system configuration, along with key accessories, including an inverse triple-resonance 1-mm probe, would enable this instrument to perform almost any state-of-the-art NMR experiment for small-molecule or biomolecular research. The requested instrumentation is intended to replace an aging, first-generation high-field 600-MHz spectrometer in the University of Iowa Central NMR facility that has seen very heavy shared usage for nearly 20 years in support of many NIH-sponsored projects. The proposed instrument would be maintained by experienced staff members, and operated primarily by individual users. Over thirty research groups on campus utilize the Central NMR facility, and over 80% of them are involved in biomedical research. The proposed instrument has been selected to fulfill current and anticipated future needs of the major NIH-sponsored users, as well as other biomedical researchers on campus. Twelve NIH-supported projects from eleven major user groups described in this application provide justification for the acquisition of the proposed spectrometer. These projects include: structural studies of bioactive natural products from fungi; synthesis, characterization, and toxicology of persistent organic pollutants; isolation and structure determination of novel oligosaccharide components of dystroglycan; analysis of isotopically-labeled versions of cofactors involved in biological electron transport systems; analysis of amino acid- and peptide-conjugates with important dopamine metabolites; molecular approaches to combat antimicrobial resistance; synthesis and structural characterization of natural products and related compounds as potential anticancer agents; structure and dynamics analysis of enzymes and signaling domains; the molecular basis of pathway selection and signaling by Nod-like receptors; and the molecular basis of calmodulin domain functions. The main themes of these projects are directed either toward the discovery of novel treatments for cancer, tuberculosis, muscular dystrophy, or Parkinson's disease, or toward expanding fundamental knowledge in other medically-related areas. Access to the state-of-the-art NMR capabilities of the proposed instrument would be beneficial to all of these efforts. PUBLIC HEALTH RELEVANCE: The requested spectrometer will be used to support research directed toward the discovery and development of new treatments for cancer, tuberculosis, malaria, and other infectious diseases. Projects dedicated to improving our molecular level understanding of muscular dystrophy, Parkinson's disease, and asthma, as well as the toxicology of environmental pollutants will also benefit from access to this instrument.