High Resolution LC-MS/MS System

描述（由申请人提供）：这是一份申请，要求提供资金购买仪器，以进行高分辨率液相色谱串联质谱（LC-MS/MS）。该仪器将位于罗伯特·伍德约翰逊医学院（RWJMS）和罗格斯大学皮斯卡特维校区现有的生物质谱设施中。高分辨率LC-MS/MS仪器将大大扩展该设施的能力，以满足31名NIH资助的研究人员日益增长的研究需求。来自NIH的资金沿着机构的支持将允许购买ThermoFisher LTQ Orbitrap XL ETD质谱仪、Proxeon纳米喷雾源和Dionex Ultimate 3000 HPLC系统。RWJMS和Rutgers没有类似的仪器。该仪器将用于表征蛋白质的翻译后修饰、定量分析/比较蛋白质组学、交联分析、溶剂可及性研究以及其他需要高分辨率LC-MS/MS功能的应用。这将加快基础和应用生物医学研究的步伐，进入重要领域，包括神经退行性疾病，传染病和癌症。公共卫生相关性：这是一个申请，要求资金购买一个专门的质谱仪，将用于由31个NIH支持的研究人员进行的项目。该仪器的可用性将加快基础和应用生物医学研究的步伐，包括神经退行性疾病，传染病和癌症。

DESCRIPTION (provided by applicant): This is an application requesting funds to purchase instrumentation to conduct high-resolution liquid chromatography tandem mass spectrometry (LC-MS/MS). The instrument will be located in an existing biological mass spectrometry facility on the Piscataway campus of Robert Wood Johnson Medical School (RWJMS) and Rutgers University. A high resolution LC-MS/MS instrument will greatly expand the capabilities of the facility to meet the increasing research needs of 31 NIH-funded investigators. Funds from NIH along with institutions support will allow the acquisition of a ThermoFisher LTQ Orbitrap XL ETD mass spectrometer, a Proxeon nanospray source, and a Dionex Ultimate 3000 HPLC system. There are no comparable instruments at RWJMS and Rutgers. The instrument will be used for characterization of post-translational modifications on proteins, quantitative analysis/comparative proteomics, crosslinking analysis, solvent accessibility studies, and other application that require high-resolution LC-MS/MS capabilities. This will accelerate the pace of basic and applied biomedical research into important areas including neurodegenerative disease, infectious disease, and cancer. PUBLIC HEALTH RELEVANCE: This is an application requesting funds to acquire a specialized mass spectrometer that will be used for projects being conducted by 31 NIH-supported investigators. The availability of this instrument will accelerate the pace of basic and applied biomedical research into important areas including neurodegenerative disease, infectious disease, and cancer.