LTQ Orbitrap Hybrid FT Mass Spectrometry System for Proteomic Analysis

描述(申请人提供)：本申请的目标是获得一种高灵敏度、高质量精度和高分辨率的质谱仪，用于复杂生物样品的蛋白质组分析。该仪器用于识别和定量翻译后修饰的氨基酸、生物多肽序列，并显著提高我们基于单一串联质谱学识别多肽或蛋白质的可信度。质谱仪将通过纳米电喷雾电离源与纳升流液相色谱系统耦合，从各种生物样品中分离和分析低丰度的多肽或蛋白质。NanoLC-MS系统将设在范德比尔特大学的质谱学资源中心，主要是为了满足NIH支持的实验室对高端质谱学日益增长的需求。这项申请旨在通过向参与生物医学研究的研究实验室提供更多高端质谱学技术，来利用范德比尔特大学丰富的蛋白质组学专业知识。正在研究的项目包括免疫反应、癌症生物学、病毒发病机制、疫苗开发、生物标记物发现、转录调控、RNA加工和翻译控制。公共卫生相关性：蛋白质组学在了解许多人类疾病的原因方面发挥着越来越重要的作用，包括癌症、炎症、关节炎和糖尿病。所要求的LTQ Orbitrap混合FT质谱学系统将为NIH资助的研究小组研究各种生物医学研究问题提供高端质谱学的途径。正在研究的项目包括免疫反应、癌症生物学、病毒发病机制、疫苗开发、生物标记物发现、转录调控、RNA加工和翻译控制。

DESCRIPTION (provided by applicant): The goal of this application is to acquire a highly sensitive mass spectrometer with high mass accuracy and high resolution for the proteomic analysis of complex biological samples. The instrument is needed to identify and quantify posttranslationally modified amino acids, sequence biological peptides, and to improve significantly our confidence in identifying a peptide or protein based on a single tandem mass spectrum. The mass spectrometer will be coupled to a nanoliter-flow liquid chromatography system via a nanoelectrospray ionization source to fractionate and analyze low-abundance peptides or proteins from a variety of biological samples. The nanoLC-mass spectrometry system will be located in Vanderbilt University's Mass Spectrometry Resource Center to primarily support the increasing demands for high-end mass spectrometry from NIH- supported laboratories. This application seeks to capitalize and leverage the tremendous proteomics expertise at Vanderbilt University by providing additional access to high-end mass spectrometry technology to research laboratories involved in biomedical research. Research projects under investigation include the immune response, cancer biology, viral pathogenesis, vaccine development, biomarker discovery, transcriptional regulation, RNA processing, and translational control. PUBLIC HEALTH RELEVANCE: Proteomics is playing an increasingly important role in understanding the causes of many human diseases including cancer, inflammation, arthritis, and diabetes. The requested LTQ Orbitrap hybrid FT mass spectrometry system will provide access to high-end mass spectrometry for NIH-funded research groups studying a variety of biomedical research issues. Projects under investigation include the immune response, cancer biology, viral pathogenesis, vaccine development, biomarker discovery, transcriptional regulation, RNA processing, and translational control.