Tandem TIme-of-Flight Mass Spectromer for Proteomics

描述(申请人提供)：本申请中描述的仪器是基质辅助激光解吸电离(MALDI)串联飞行时间(TOF/TOF)质谱仪。拟议的仪器将是共享质谱学用户设施的一部分，设在路易斯安那州立大学基础科学学院化学系。该设施由在质谱学和分离方面拥有丰富经验的博士科学家担任工作人员和监督人员。这一工具的使用将大大提高NIH支持的大量调查人员的质量、数量和范围。该仪器的使用将得到加强的具体项目包括基础科学学院生物科学系：该仪器将用于分析未修饰的铁硫蛋白(Ding)、在肝脏代谢中重要的蛋白质/蛋白质相互作用(Pr|FER)以及鉴定STAT蛋白质信号转导通路中涉及的蛋白质(Stephens)。基础科学学院化学系：MALDI TOF/TOF仪器将通过开发和应用基于毛细管电泳法的方法研究酶抑制(Gilman)、抗阿尔茨海默氏症多肽类似物的开发研究(Hammer)、MS/MS细菌生物标记物的鉴定(Murray)、用于生物成像和癌症治疗的卟啉生物偶联物研究(Vicente)以及参与动脉粥样硬化斑块形成的蛋白质的结构分析(Warner)，支持酶功能的研究。路易斯安那州立大学兽医学院：建议的仪器将用于在HSV-1膜融合蛋白的遗传学和功能研究中实现最大序列覆盖，MALDI与ESI并行运行，LC分离消化肽(Kousoulas)。拟议的仪器将补充已在MS设施中的混合四极TOF质谱仪，并将能够利用现有的研究基础设施来进行LC-MALDI板定位。公共卫生相关性：相关性质谱仪是分析生物分子的强大工具，获得它们对于生物医学研究的成功至关重要。提出的仪器具有高质量分辨率、大质量范围和高吞吐量的独特能力，这是路易斯安那州立大学的研究人员目前无法获得的。该仪器将使国家卫生研究院资助的路易斯安那州立大学的研究人员能够识别和量化蛋白质和其他生物大分子，这些蛋白质和生物大分子对了解和抗击疾病以及保护公众健康至关重要。该仪器将由熟练的技术人员操作，由具有广泛代表性的委员会监督，并可供整个路易斯安那州立大学科学界使用。

DESCRIPTION (provided by applicant): The instrument described in this application is a matrix-assisted laser desorption ionization (MALDI) tandem time-of-flight (TOF/TOF) mass spectrometer. The proposed instrument will be a part of the shared mass spectrometry user facility and housed in the College of Basic Sciences Department of Chemistry at Louisiana State University. This facility is staffed and supervised by Ph.D. scientists with extensive experience in mass spectrometry and separations. Access to this instrument will significantly enhance the quality, quantity and scope of a large number of NIH supported investigators. Specific projects that will be enhanced by access to this instrument include College of Basic Sciences, Biological Sciences Department: The instrument will be used in analysis of NO-modified iron-sulfur proteins (Ding), protein/protein interactions important in liver metabolism (Pr|fer), and the identification of proteins involved in STAT protein signal transduction pathways (Stephens). College of Basic Sciences, Chemistry Department: the MALDI TOF/TOF instrument will support the study of enzyme function through the development and application of capillary electrophoresis-based approaches for studying enzyme inhibition (Gilman), research in the development of anti-Alzheimer's peptide analogs (Hammer), the identification of MS/MS bacterial biomarkers (Murray), the study of porphyrin bioconjugates for bioimaging and cancer therapy (Vicente), and the structural analysis of proteins involved in atherosclerotic plaque formation (Warner). Louisiana State University School of Veterinary Medicine: The proposed instrument will be used to achieve maximum sequence coverage in a study of genetics and functions of the HSV-1 membrane fusion protein with MALDI run in parallel with ESI with LC separation of digested peptides (Kousoulas). The proposed instrument will complement a hybrid quadrupole TOF mass spectrometer already in the MS facility and will be able to leverage existing research infrastructure for LC-MALDI plate spotting. PUBLIC HEALTH RELEVANCE: Relevance Mass spectrometers are powerful tools of the analysis of biomolecules and access to them is essential for the success of biomedical research. The proposed instrument has unique capabilities of high mass resolution, large mass range, and high throughput that are not currently available to researchers at Louisiana State University. This instrument will allow NIH funded researchers at LSU to identify and quantify proteins and other biomacromolecules important in understanding and combating diseases and in protecting the public health. The instrument will be operated by skilled technicians, overseen by broadly represented committee and available for use by the entire LSU scientific community.