Leica TCS SP5 laser scanning confocal microscope

描述（由申请人提供）：Linser，Paul J.确保惠特尼实验室显微镜能力的连续性佛罗里达大学惠特尼实验室是一个致力于细胞和分子生物学的研究所。常驻教师调查广泛的模型系统和细胞生物学的基本问题。该研究所大多数研究的一个自然部分是使用先进的显微技术分析细胞功能和细胞功能定位。激光扫描共聚焦显微镜已成为这些分析的许多方面不可或缺的工具。惠特尼实验室的老化（7岁）Leica LSCM SP2不再支持新零件的制造，导致停机时间越来越长。此外，SP2的原始设计在当前研究环境中的局限性为惠特尼实验室的许多具体研究造成了技术障碍。该拨款提案是共享仪器拨款（SIG），为购买新的最先进的Leica TCS SP5共聚焦显微镜提供资金。在过去的七年里，这项技术在硬件和软件方面都取得了重大进展，这为研究提供了许多新的机会。与过时的SP2相关的频繁故障周期配对，更换显微镜是惠特尼实验室研究所长期成就历史的持续成功不可或缺的。公共卫生相关性：该提案旨在为惠特尼实验室的Leica TCS SP5激光扫描共聚焦显微镜提供资金。惠特尼实验室是一个前沿的生物医学实验室，30多年来在基础细胞生物学、神经科学和公共卫生等研究领域发表了许多突破性的出版物。技术上的并行性（例如共聚焦显微镜）对于佛罗里达大学惠特尼实验室继续为生物医学做出贡献至关重要。

DESCRIPTION (provided by applicant): Linser, Paul J. Ensuring the Continuity of the Whitney Laboratory's Microscopy Capacity The Whitney Laboratory of the University of Florida is a research Institute dedicated to cell and molecular biology. The resident faculty members investigate a wide range of model systems and fundamental questions in cell biology. A natural part of most investigations at the Institute is analysis of cell function and cell functionality localization using advanced microscopic techniques. A laser scanning confocal microscope has become an integral and indispensable tool in many aspects of these analyses. The Whitney Lab's aging (7 year old) Leica LSCM SP2 is no longer supported with the manufacture of new parts which has lead to increasingly lengthy periods of downtime. Furthermore, the limitations on the original design of the SP2 in the current research climate creates technical hurdles to many specific investigations at the Whitney Lab. This grant proposal is for a Shared Instrumentation Grant (SIG) to provide funds for the purchase of a new, state-of-the-art Leica TCS SP5 confocal microscope. Major advances have occurred in both hardware and software in the past seven years for this technology which opens many new research opportunities. Paired with the frequent periods of malfunction associated with the out-dated SP2, a replacement microscope is integral to the continued success of the Whitney Lab Research Institute's long history of accomplishment. PUBLIC HEALTH RELEVANCE: This proposal is to provide funding for a Leica TCS SP5 laser scanning confocal microscope at the Whitney Laboratory. The Whitney Lab is a cutting edge Biomedical Laboratory and for over 30 years has provided numerous breakthrough publications in areas of research including fundamental cell biology, neuroscience and public health. Technical concurrency (e.g. confocal microscope) is crucial to continued contributions to biomedicine made by the Whitney Laboratory of the University of Florida.