ANION REGULATION IN LARVAE OF DISEASE VECTOR MOSQUITOES

决策(改编自申请人的摘要)：长期 本项目的目标是分离、鉴定和克隆碳素 该病的脱水酶和氯离子/碳酸氢根阴离子交换器 媒介蚊子，埃及伊蚊，运输模型毛虫， 曼杜卡六种。这两种酶在中肠碱化中的作用 并将测定离子动态平衡和阴离子缓冲模型 制定管理办法。分离的蛋白scDNA将被用于 制备用于细胞定位的免疫学和分子探针 分析。最重要的是它独特的高碱度 中肠内容物是通过H+V-ATPase超极化实现的 通过电泳法将流明H+交换为细胞K+， 衬底驱动的流明氯离子与HCO3-的交换，以及电压驱动 除去H+生成CO3-。因为，碳酸酐酶和阴离子 交换抑制可能是小说的有吸引力的候选者， 对环境安全，杀幼虫。

DECSRIPTION (Adapted from the Applicant's Abstract): The long term objective of this project is to isolate, characterize and clone carbonic anhydrase and chloride/bicarbonate anion exchanger from the disease vector mosquito, Aedes aegypti, and the transport model caterpillar, Manduca sexta. The role of theses two enzymes in midgut alkalinzation and ionic homeostasis will be determined and models of anionic buffer regulation will be formulated. The isolated proteinscDNAs will be used to prepare immunological and molecular probes for cellular localization analyses. The hupothesies is that the uniquely high alkalinity of the midgut contents is achieved across an H+ V-ATPase-hyperpolarization membrane by electrophoretic exchange of luminal H+ for cell K+, substrate-driven exchange of luminal Cl- for HCO3-, and voltagew driven removal of H+ to yield CO3--. Since, carbonic anhydrase and anion exchanger inhibition maqy be attractive candidates for novel, environmentally safe, larvacides.