ANION REGULATION IN LARVAE OF DISEASE VECTOR MOSQUITOES

DECSRIPTION（改编自申请人摘要）：长期 本项目的目标是分离、表征和克隆碳 脱水酶和氯/碳酸氢根阴离子交换剂 媒介蚊子，埃及伊蚊，和运输模型毛虫， 曼陀罗这两种酶在中肠碱化中的作用 和离子稳态将被确定和模型的阴离子缓冲液 将制定法规。将使用分离的蛋白质scDNAs 制备用于细胞定位的免疫学和分子探针 分析。这种独特的高碱性 中肠内容物通过H+ V-ATP酶超极化实现 膜通过电泳交换管腔H+为细胞K+， 底物驱动鲁米诺Cl-交换为HCO 3-，电压驱动 除去H+生成CO 3--。由于碳酸酐酶和阴离子 交换抑制可能是新颖的， 对环境无害的杀幼虫剂

DECSRIPTION (Adapted from the Applicant's Abstract): The long term objective of this project is to isolate, characterize and clone carbonic anhydrase and chloride/bicarbonate anion exchanger from the disease vector mosquito, Aedes aegypti, and the transport model caterpillar, Manduca sexta. The role of theses two enzymes in midgut alkalinzation and ionic homeostasis will be determined and models of anionic buffer regulation will be formulated. The isolated proteinscDNAs will be used to prepare immunological and molecular probes for cellular localization analyses. The hupothesies is that the uniquely high alkalinity of the midgut contents is achieved across an H+ V-ATPase-hyperpolarization membrane by electrophoretic exchange of luminal H+ for cell K+, substrate-driven exchange of luminal Cl- for HCO3-, and voltagew driven removal of H+ to yield CO3--. Since, carbonic anhydrase and anion exchanger inhibition maqy be attractive candidates for novel, environmentally safe, larvacides.