Anion Regulation in Larvae of Disease Vector Mosquitoes

描述：(申请人提供)：长期目标是 描述幼虫蚊子上皮细胞的主要功能 肠道作为这些细胞功能影响和调节阴离子基础 肠腔的碱化。详细了解肠道上皮细胞如何 细胞产生非凡的和生物上独特的极端pH，推动 消化过程(即&gt；10.5)将为 环境安全和特定的杀幼虫剂。有两个特定的基因家族 成为目标，因为它们在阴离子的产生和运输中发挥着核心作用 肠道碱化过程：碳酸氢酶和跨膜阴离子 传送者。这个项目将产生分子和生理 这两个基因家族成员的特征。它们的分布在 蚊子幼虫肠道碱化的组织和特殊作用将是 已定义。整个肠道的特定细胞表型将被定义并 评估了每种物质在碱化过程中的作用。目标1将研究 多种碳酸酐酶的表达。目标2将定义和描述 阴离子转运蛋白基因家族的成员。目标3将定义细胞 碳酸氢酶和阴离子转运体在肠道和动脉粥样硬化中的分布 幼虫发育的功能。目标4将带来特定的本地化 基因产品及其活性的生理测量 单个细胞产生细胞特异性和阴离子的空间分析 肠道上皮细胞的动力学。因为蚊子是人类面临的头号威胁 在人类健康的世界范围内，制定新的控制策略 它们生物学的独特方面(即肠道碱化)具有重要的意义 潜力。

DESCRIPTION: (provided by the applicant): The long term objective is to characterize key functionalities of the epithelial cells of the larval mosquito gut as these cellular functions influence and regulate the anionic basis of alkalinization of the gut lumen. A detailed understanding of how gut epithelial cells produce the remarkable and biologically unique pH extremes that drive the digestive process (i.e. >10.5) will provide new avenues for the development of environmentally safe and specific larvacides. Two specific gene families have been targeted as they have central roles in anion production and transport in the gut alkalinization process: carbonic anhydrases and transmembrane anion transporters. This project will produce molecular and physiological characterizations of members of these two gene families. Their distributions in the tissue and specific roles in larval mosquito gut alkalinization will be defined. Specific cellular phenotypes throughout the gut will be defined and the role of each in the alkalinization process assessed. AIM 1 will examine the expression of multiple carbonic anhydrases. AIM 2 will define and characterize members of the anion transporter gene family. AIM 3 will define the cellular distributions of carbonic anhydrases and anion transporters in the gut and as functions of larval development. AIM 4 will bring the localization of specific gene products together with physiological measurements of the activity of individual cells to produce a cell-specific and spatial analysis of anion dynamics in the gut epithelium. As mosquitoes are the number one threat to human health world wide, the development of new strategies for control based on unique aspects of their biology (i.e., gut alkalinization) has important potential.