Anion Regulation in Larvae of Disease Vector Mosquitoes

描述：（由申请人提供）：长期目标是 描述蚊子幼虫上皮细胞的关键功能 肠道作为这些细胞功能的影响和调节阴离子的基础， 肠腔碱化。详细了解肠道上皮细胞 细胞产生显著的和生物学上独特的pH极端， 消化过程（即>10.5）将为发展提供新的途径， 对环境安全和特定的杀幼虫剂。两个特定的基因家族 由于它们在阴离子的产生和运输中发挥着核心作用， 肠道碱化过程：碳酸酐酶和跨膜阴离子 运输机该项目将产生分子和生理 这两个基因家族的成员的特征。分布在 幼虫蚊子肠道碱性化的组织和具体作用将 定义了将定义整个肠道的特定细胞表型， 在碱化过程中的每一个评估的作用。AIM 1将检查 多种碳酸酐酶的表达。AIM 2将定义和表征 阴离子转运蛋白基因家族的成员。AIM 3将定义蜂窝 碳酸酐酶和阴离子转运蛋白在肠道中的分布， 幼虫发育的功能。AIM 4将带来特定的本地化 基因产物与生理测量的活性， 单个细胞以产生阴离子的细胞特异性和空间分析 肠道上皮的动力学。由于蚊子是人类的头号威胁， 全世界的人类健康，发展新的控制战略， 其生物学的独特方面（即，肠道碱化）具有重要的 潜力

DESCRIPTION: (provided by the applicant): The long term objective is to characterize key functionalities of the epithelial cells of the larval mosquito gut as these cellular functions influence and regulate the anionic basis of alkalinization of the gut lumen. A detailed understanding of how gut epithelial cells produce the remarkable and biologically unique pH extremes that drive the digestive process (i.e. >10.5) will provide new avenues for the development of environmentally safe and specific larvacides. Two specific gene families have been targeted as they have central roles in anion production and transport in the gut alkalinization process: carbonic anhydrases and transmembrane anion transporters. This project will produce molecular and physiological characterizations of members of these two gene families. Their distributions in the tissue and specific roles in larval mosquito gut alkalinization will be defined. Specific cellular phenotypes throughout the gut will be defined and the role of each in the alkalinization process assessed. AIM 1 will examine the expression of multiple carbonic anhydrases. AIM 2 will define and characterize members of the anion transporter gene family. AIM 3 will define the cellular distributions of carbonic anhydrases and anion transporters in the gut and as functions of larval development. AIM 4 will bring the localization of specific gene products together with physiological measurements of the activity of individual cells to produce a cell-specific and spatial analysis of anion dynamics in the gut epithelium. As mosquitoes are the number one threat to human health world wide, the development of new strategies for control based on unique aspects of their biology (i.e., gut alkalinization) has important potential.