Coherent AntiStokes Raman Scattering Microscope

描述（由申请人提供）：我们建议购买相干反斯托克斯拉曼散射显微镜系统。该仪器提供了生物样品和组织的新型分子特异性无标记成像。特别地，该系统将与活体显微镜方法兼容，并且将是可调的，使得它可以检测同一样品中的多个分子。参与NIH临床和基础研究的广泛研究人员建议使用该系统来增强和发展他们的兴趣。这些单独的项目利用汽车为成像带来的全部好处。这些包括无标记非侵入性成像，与活细胞和活体显微镜兼容。该系统中使用的NIR激光源特别适合于组织成像。深度分辨成像是汽车技术中固有的。这允许以亚微米分辨率映射三维组织或样本体积。汽车显微镜已经被各种各样的研究人员指定用于一系列疾病相关的问题。汽车在成像脂质沉积和脂质动力学方面的优势已被该小组充分利用。此外，我们还提出了其在成像钙化，如在脉管系统中的使用。汽车系统为基础科学和医学的研究能力增加了新的广度。它在医学研究校园的位置确保了其有效和富有想象力的使用。公共卫生相关性：汽车显微镜是一种新的方法，用于成像细胞和组织中发生的靶分子的三维分布，而无需将外部标记物应用于这些分子。活细胞和组织的成像是可能的，这反过来又导致对这些分子在动态生理过程和疾病中的作用的新理解。

DESCRIPTION (provided by applicant): We propose to purchase a Coherent AntiStokes Raman Scattering Microscopy system. This instrument offers novel molecule specific label free imaging of biological samples and tissue. In particular this system will be compatible with intravital microscopy approaches and will be tunable such that it can detect multiple molecules in the same sample. A wide range of investigators involved in NIH clinical and basic research propose to use the system to enhance and develop their interests. These individual projects exploit the full range of benefits that CARS brings to imaging. These include label free non invasive imaging, compatibility with live cell and intravital microscopy. The NIR laser source used in this system is particularly suitable for tissue imaging. Depth resolved imaging is inherent in the CARS technique. This allows mapping of three dimensional tissue or sample volumes with submicron resolution. The CARS microscope has been specified by a diverse range of investigators working on a range of disease related problems. The strength of CARS in imaging lipid deposits and lipid dynamics has been fully exploited by this group. In addition we also propose its use in imaging calcifications such as in the vasculature. The CARS system adds new breadth to research capabilities in the basic sciences and medicine. Its location on a medical research campus ensures its effective and imaginative use. PUBLIC HEALTH RELEVANCE: CARS microscopy is a new method for imaging the three dimensional distribution of target molecules occurring in cells and tissue without the needing to apply an external marker to those molecules. Imaging of live cells and tissues is possible which in turn leads to new understanding of the role of these molecules in dynamic physiological processes and in disease.