CCD Camera for Cryo-EM

描述(由申请人提供)：我们需要一台4K乘4K的摄像机，专为冷冻EM而设计。该相机将安装在JEOL JEM2010 FasTEM/STEM显微镜上。冷冻EM设备致力于研究生物大分子的3D结构。这些蛋白质复合体在人类健康和疾病中起着至关重要的作用。该显微镜位于布鲁克海文国家实验室，由BNL和石溪大学生物化学和细胞生物系联合任命的李慧琳操作。大幅面相机及其相关软件将使显微镜自动化和低温透射电子显微镜和低温STEM双峰层析成像成为可能，从而显著提高显微镜产量和研究生产率。在纽约长岛，有三个大型研究所进行NIH支持的研究。这就是所谓的长岛三学院：中间是纽约州立大学石溪分校，东面约15英里处是布鲁克海文国家实验室，西面约15英里处是冷泉港实验室。当地研究人员对大型生物组件的低温电磁成像有许多需求。然而，我们的冷冻-EM小组是长岛唯一可用的设施。所要求的仪器将促进现有的NIH资助的低温EM项目，并有可能容纳更多来自当地生物医学界的项目。 公共卫生相关性：我们需要一台专为在电子显微镜中记录数字图像而设计的CCD摄像机。该显微镜设备致力于研究生物大分子的三维结构。这些蛋白质复合体在人类健康和疾病中起着至关重要的作用。大幅面相机及其相关软件将实现显微镜自动化，并显著提高显微镜产量和研究生产率。

DESCRIPTION (provided by applicant): We request a 4K by 4K CCD camera designed for cryo-EM. The camera will be installed in a JEOL JEM2010 FasTEM/STEM microscope. The cryo-EM facility is dedicated to studying the 3D structures of biological macromolecules. These protein complexes play critical roles in human health and disease. The microscope is located in Brookhaven National Lab and operated by Huilin Li, who holds a joint appointment between BNL and Biochemistry and Cell Biology Department in Stony Brook University. The large format camera and its associated software will enable microscopy automation and cryo-TEM and cryo-STEM bimodal tomography, thus significantly increase the microscopy throughput and research productivity. There are three large institutes on Long Island, New York, that conduct NIH-supported research. These are the so-called Long Island Tri-Institutes: SUNY Stony Brook being in the middle, Brookhaven National Laboratory about 15 miles to the east, and Cold Spring Harbor Laboratory about 15 miles to the west. There are numerous needs for cryo-EM imaging of large biological assemblies from local researchers. However, our cryo-EM group is the only facility available on Long Island. The requested instrument will facilitate existing NIH-funded cryo-EM projects, with the likelihood of accommodating several more projects from local biomedical community. Public Health Relevance: We request a CCD camera designed for recording digital images in an electron microscope. The microscopy facility is dedicated to studying the 3D structures of biological macromolecules. These protein complexes play critical roles in human health and disease. The large format camera and its associated software will enable microscopy automation and significantly increase the microscopy throughput and research productivity.