Illumina Genome Analyzer II

描述（由申请人提供）：本申请申请资助Illumina Solexa高通量测序装置，以加强和扩展加州大学圣地亚哥分校现有的生物医学基因组学微阵列设施（BIOGEM）。BIOGEM是由医学院和UCSD癌症中心（nci指定的综合癌症中心）共同管理的校园核心设施。该申请主要关注中心一组癌症生物学家在后基因组时代追求前沿研究的需求。利用加州大学圣地亚哥分校HHMI实验室现有的Solexa测序仪，我们已经建立了几个标准的应用程序，使用该仪器进行基因表达的数字分析和功能性DNA元件的基因组定位。我们还在开发其他基于高通量测序的技术，用于绘制功能性RNA元件，探测基因网络，以及检测癌症中的基因和染色体重排。由于现有HHMI单元的访问有限，我们将把Solexa安置在BIOGEM设施中，以允许各种数字技术应用于特定的癌症生物学问题，目前正在资助请求中引用的主要用户的实验室中进行调查。公共卫生相关性：本提案寻求购买Solexa仪器的资金，这是Illumina公司最新的高通量测序系统。加州大学圣地亚哥分校的一组癌症生物学家将利用该仪器来解决一系列由美国国立卫生研究院资助的研究癌症相关问题的项目，这些项目对癌症预防和治疗策略的发展至关重要。

DESCRIPTION (provided by applicant): This application requests funding for an Illumina Solexa high-throughput sequencing unit to strengthen and expand the existing the Biomedical Genomics Microarray Facility (BIOGEM) at the University of California, San Diego. BIOGEM is a campus core facility managed jointly by the School of Medicine and the Moores UCSD Cancer Center, an NCI-designated comprehensive cancer center. This application focuses on the need for a group of cancer biologists in the Center to pursue cutting-edge research in the post-genomic era. Taking advantage of the existing Solexa sequencer in a HHMI lab at UCSD, we have established several standard applications using the instrument for digital analysis of gene expression and genomic mapping of functional DNA elements. We are also developing other high-throughput sequencing-based technologies for mapping functional RNA elements, probing gene networks, and detecting gene and chromosomal rearrangements in cancers. Because of the limited access to the existing HHMI unit, we will house the Solexa in the BIOGEM facility, to allow applications of various digital technologies to specific cancer biology problems now under investigation in the laboratories of the prime users cited in the grant request. PUBLIC HEALTH RELEVANCE: This proposal seeks funding for the purchase of a Solexa instrument, the latest high- throughput sequencing system from Illumina. The instrument will be utilized by a group of UCSD cancer biologists to address an array of NIH-funded projects studying a variety of cancer-related problems, which are essential for development of strategies for cancer prevention and therapeutics.