Mouse Core

我们推出鼠标内核的初衷是为了创造一个与其他鼠标内核截然不同的内核。我们的首要目标是利用小鼠核心来产生新的转基因和基因靶向的小鼠品系，这将使我们能够作为一个群体进行测试， 内耳生物学的基本问题。因此，我们打算让小鼠核心在结构设计和通用、小鼠饲养和基因分型方面拥有专业知识。 第一次续订提交的审查员很容易就注意到一个不可否认的事实，即我们在制造新的鼠标线方面远远没有我们参与的成功。我们的初衷是通过产生小鼠系来播种构建-生成过程 这些建议与核心非政府组织的利益更密切相关，但却触及了人权高专办小组普遍感兴趣的问题，如机械转导。 然而，小鼠核心在构建世代和获得生殖系方面停滞不前 传输和它的构造。我们现在可以开始在小组之间共享两个生成的小鼠品系(TRPV5和TRPV6敲击)，但我们更愿意几年前在这里。 此外，鼠标核心科学拳击手吉姆·斯马特能够管理核心的方方面面，他离开了，去了一个更稳定的职位，一个有长期就业保障的职位。我们很幸运地以我们付给他的薪水得到了他，因为他很有经验；我们 他刚从研究生院毕业就被选中，能够给他足够的报酬，让这个职位看起来很有吸引力。然而，这不是这个职位的长期解决方案。这一经验确实表明，聘请有制作经验的人 用可用的P30预算，鼠标线不是很容易完成的。 然而，我们最终聘请了一名Mouse Core技术人员Michael Bateschell，他在面向服务的核心方面表现出了出色的技能。结合保留Bateschell的需要和将核心扩展到包括在子宫电穿孔中的计划，非常明显的是，我们无法为核心聘请具有足够分子遗传学经验的人来继续构建一代 服务。在第一次提交续期报告后对这一核心的负面审查正好强化了这一观点。

Our original intent with the Mouse Core was to create a core that was rather different than other mouse cores. Our overarching goal was to use the Mouse Core to generate new transgenic and gene-targeted mouse lines that would allow us to test, as a group, fundamental problems in inner-ear biology. We intended therefore for the Mouse Core to have experfise in construct design and generafion, mouse husbandry, and genotyping. The reviewers of the first renewal submission easily picked up on the undeniable fact that we were far less successful than we had anficipated in making new mouse lines. Our original intent was to seed the construct-generation process by generating mouse lines that were more closely related to the interests of the Core PI, but that nevertheless tapped into topics of general interest to the OHRC group, such as mechanotransduction. However, the Mouse Core bogged down with construct generation and in obtaining germline transmission with its constructs. We are now at a position where we can begin to share the two generated mouse lines (TRPV5 and TRPV6 knock-ins) amongst the group, but we would have preferred to be here a few years ago. Moreover, the Mouse Core Scienfist, Jim Smart, who was capable of running all facets of the core, left for a more stable posifion, one with a long-term guarantee of employment. We had been lucky to get him at the salary we paid him, as he was quite experienced; we picked him straight out of graduate school, and were able to give him sufficient compensafion for the position to look attractive. That was not a long-term solufion for this position, however. This experience did point out that hiring someone with experience in making mouse lines was not easily done with the available P30 budget. In the meanfime, however, we had hired a Mouse Core Technician, Michael Bateschell, who demonstrated outstanding skill in the service-oriented aspects of the core. Combining the need to retain Bateschell and the plan to expand the core to included in utero electroporation, it became abundantly clear that we would not be able to hire someone for the core with sufficient molecular-genetics experience to be able to continue with the construct-generation service. The negative review of this core after its first renewal submission just reinforced this view.